Investigation of the relationships between plasma levels of ascorbate, vitamin E and beta-carotene and the frequency of sister-chromatid exchanges and release of reactive oxidants by blood leucocytes from cigarette smokers

Mutat Res. 1989 Dec;215(2):167-72. doi: 10.1016/0027-5107(89)90180-2.

Abstract

In this study the frequencies of sister-chromatid exchanges (SCEs) were correlated with measurements of the release of reactive oxidants by phagocytes, as determined by luminol-enhanced chemiluminescence (LECL), and levels of the anti-oxidants ascorbate, beta-carotene and vitamin E in blood specimens taken from 65 young asymptomatic cigarette smokers. Increased SCE frequencies correlated with LECL responses (p less than 0.0075) of activated blood phagocytes. Anti-oxidant levels did not correlate with either LECL or SCEs. These findings indicate that increased generation of reactive oxidants by circulating phagocytes from cigarette smokers are associated with cytogenetic changes.

MeSH terms

  • Adult
  • Age Factors
  • Ascorbic Acid / blood*
  • Carotenoids / blood*
  • Female
  • Free Radicals
  • Humans
  • Leukocytes / physiology*
  • Luminescent Measurements
  • Lymphocytes / ultrastructure
  • Male
  • N-Formylmethionine Leucyl-Phenylalanine / pharmacology
  • Oxygen / metabolism*
  • Phagocytes / physiology
  • Sex Factors
  • Sister Chromatid Exchange*
  • Smoking / blood*
  • Vitamin E / blood*
  • beta Carotene

Substances

  • Free Radicals
  • beta Carotene
  • Vitamin E
  • Carotenoids
  • N-Formylmethionine Leucyl-Phenylalanine
  • Ascorbic Acid
  • Oxygen