Phlebotomus papatasi SP15: mRNA expression variability and amino acid sequence polymorphisms of field populations

Marcelo Ramalho-Ortigão; Iliano V Coutinho-Abreu; Valdir Q Balbino; Carlos Alberto S Figueiredo Jr; Rami Mukbel; Hussan Dayem; Hanafi A Hanafi; Shabaan S El-Hossary; Emad El-Din Y Fawaz; Mahmoud Abo-Shehada; David F Hoel; Gwen Stayback; Mariha Wadsworth; Douglas A Shoue; Jenica Abrudan; Neil F Lobo; Andrew R Mahon; Scott J Emrich; Shaden Kamhawi; Frank H Collins; Mary Ann McDowell

doi:10.1186/s13071-015-0914-2

Phlebotomus papatasi SP15: mRNA expression variability and amino acid sequence polymorphisms of field populations

Parasit Vectors. 2015 May 29:8:298. doi: 10.1186/s13071-015-0914-2.

Authors

Marcelo Ramalho-Ortigão¹, Iliano V Coutinho-Abreu², Valdir Q Balbino³, Carlos Alberto S Figueiredo Jr⁴, Rami Mukbel⁵, Hussan Dayem⁶, Hanafi A Hanafi⁷, Shabaan S El-Hossary⁸, Emad El-Din Y Fawaz⁹, Mahmoud Abo-Shehada¹⁰, David F Hoel¹¹, Gwen Stayback¹², Mariha Wadsworth¹³, Douglas A Shoue¹⁴, Jenica Abrudan¹⁵, Neil F Lobo¹⁶, Andrew R Mahon¹⁷, Scott J Emrich^{18

19}, Shaden Kamhawi²⁰, Frank H Collins^{21

22}, Mary Ann McDowell²³

Affiliations

¹ Department of Entomology, Kansas State University, Manhattan, KS, 66506, USA. [email protected].
² Laboratory of Malaria and Vector Research, NIAID-NIH, 12735 Twinbrook Parkway, Rockville, MD, 20852, USA. [email protected].
³ Department of Genetics, Universidade Federal de Pernambuco, Recife, PE, Brazil. [email protected].
⁴ Department of Genetics, Universidade Federal de Pernambuco, Recife, PE, Brazil. [email protected].
⁵ Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, 22110, Jordan. [email protected].
⁶ Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, 22110, Jordan. [email protected].
⁷ Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, 22110, Jordan. [email protected].
⁸ Vector Biology Research Program, U.S. Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt. [email protected].
⁹ Vector Biology Research Program, U.S. Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt. [email protected].
¹⁰ Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, 22110, Jordan. [email protected].
¹¹ Department of Biostatistics, Uniformed Services University of the Health Sciences, Bethesda, MD, 20814, USA. [email protected].
¹² Eck Institute for Global Health, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, 46556, USA. [email protected].
¹³ Eck Institute for Global Health, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, 46556, USA. [email protected].
¹⁴ Eck Institute for Global Health, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, 46556, USA. [email protected].
¹⁵ Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, Philadelphia, USA. [email protected].
¹⁶ Eck Institute for Global Health, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, 46556, USA. [email protected].
¹⁷ Department of Biology, Central Michigan University, Mount Pleasant, Detroit, MI, 48859, USA. [email protected].
¹⁸ Eck Institute for Global Health, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, 46556, USA. [email protected].
¹⁹ Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN, 46556, USA. [email protected].
²⁰ Laboratory of Malaria and Vector Research, NIAID-NIH, 12735 Twinbrook Parkway, Rockville, MD, 20852, USA. [email protected].
²¹ Eck Institute for Global Health, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, 46556, USA. [email protected].
²² Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN, 46556, USA. [email protected].
²³ Eck Institute for Global Health, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, 46556, USA. [email protected].

Abstract

Background: The Phlebotomus papatasi salivary protein PpSP15 was shown to protect mice against Leishmania major, suggesting that incorporation of salivary molecules in multi-component vaccines may be a viable strategy for anti-Leishmania vaccines.

Methods: Here, we investigated PpSP15 predicted amino acid sequence variability and mRNA profile of P. papatasi field populations from the Middle East. In addition, predicted MHC class II T-cell epitopes were obtained and compared to areas of amino acid sequence variability within the secreted protein.

Results: The analysis of PpSP15 expression from field populations revealed significant intra- and interpopulation variation.. In spite of the variability detected for P. papatasi populations, common epitopes for MHC class II binding are still present and may potentially be used to boost the response against Le. major infections.

Conclusions: Conserved epitopes of PpSP15 could potentially be used in the development of a salivary gland antigen-based vaccine.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Amino Acid Sequence
Animals
Genetic Variation
Insect Proteins / chemistry
Insect Proteins / genetics*
Insect Proteins / metabolism
Molecular Sequence Data
Phlebotomus / chemistry
Phlebotomus / genetics*
Phlebotomus / metabolism
Polymorphism, Genetic
RNA, Messenger / genetics
RNA, Messenger / metabolism
Sequence Alignment

Substances

Insect Proteins
RNA, Messenger

Abstract

Publication types

MeSH terms

Substances

Grants and funding