Abstract
Galacto-N-biose (GNB) derivatives were efficiently synthesized from galactose derivatives via a one-pot two-enzyme system containing two promiscuous enzymes from Bifidobacterium infantis: a galactokinase (BiGalK) and a d-galactosyl-β1-3-N-acetyl-d-hexosamine phosphorylase (BiGalHexNAcP). Mono-sialyl and di-sialyl galacto-N-biose derivatives were then prepared using a one-pot two-enzyme system containing a CMP-sialic acid synthetase and an α2-3-sialyltransferase or an α2-6-sialyltransferase.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Bifidobacterium / enzymology
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Disaccharides / chemical synthesis*
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Galactans / chemical synthesis*
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Galactokinase / chemistry
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Galactokinase / metabolism
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Galactosyltransferases / chemistry
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Galactosyltransferases / metabolism
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N-Acylneuraminate Cytidylyltransferase / chemistry
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N-Acylneuraminate Cytidylyltransferase / metabolism
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Sialic Acids / chemical synthesis*
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Sialyltransferases / chemistry
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Sialyltransferases / metabolism
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beta-D-Galactoside alpha 2-6-Sialyltransferase
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beta-Galactoside alpha-2,3-Sialyltransferase
Substances
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Disaccharides
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Galactans
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Sialic Acids
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Galactosyltransferases
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beta-1,3-galactosyl-N-acetylhexosamine phosphorylase
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Sialyltransferases
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Galactokinase
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N-Acylneuraminate Cytidylyltransferase
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beta-D-Galactoside alpha 2-6-Sialyltransferase
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beta-Galactoside alpha-2,3-Sialyltransferase