Inactivation of Escherichia coli Cells in Aqueous Solution by Atmospheric-Pressure N2, He, Air, and O2 Microplasmas

Appl Environ Microbiol. 2015 Aug;81(15):5257-65. doi: 10.1128/AEM.01287-15. Epub 2015 May 29.

Abstract

Atmospheric-pressure N2, He, air, and O2 microplasma arrays have been used to inactivate Escherichia coli cells suspended in aqueous solution. Measurements show that the efficiency of inactivation of E. coli cells is strongly dependent on the feed gases used, the plasma treatment time, and the discharge power. Compared to atmospheric-pressure N2 and He microplasma arrays, air and O2 microplasma arrays may be utilized to more efficiently kill E. coli cells in aqueous solution. The efficiencies of inactivation of E. coli cells in water can be well described by using the chemical reaction rate model, where reactive oxygen species play a crucial role in the inactivation process. Analysis indicates that plasma-generated reactive species can react with E. coli cells in water by direct or indirect interactions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Air
  • Atmospheric Pressure*
  • Escherichia coli / drug effects*
  • Helium / toxicity
  • Microbial Viability / drug effects*
  • Nitrogen / toxicity
  • Oxygen / toxicity
  • Plasma Gases / toxicity*
  • Reactive Oxygen Species / toxicity
  • Water Microbiology

Substances

  • Plasma Gases
  • Reactive Oxygen Species
  • Helium
  • Nitrogen
  • Oxygen