Discovery of a Novel Polymer for Human Pluripotent Stem Cell Expansion and Multilineage Differentiation

Adam D Celiz; James G W Smith; Asha K Patel; Andrew L Hook; Divya Rajamohan; Vinoj T George; Luke Flatt; Minal J Patel; Vidana C Epa; Taranjit Singh; Robert Langer; Daniel G Anderson; Nicholas D Allen; David C Hay; David A Winkler; David A Barrett; Martyn C Davies; Lorraine E Young; Chris Denning; Morgan R Alexander

doi:10.1002/adma.201501351

Discovery of a Novel Polymer for Human Pluripotent Stem Cell Expansion and Multilineage Differentiation

Adv Mater. 2015 Jul 15;27(27):4006-12. doi: 10.1002/adma.201501351. Epub 2015 Jun 1.

Authors

Adam D Celiz^{1

2}, James G W Smith³, Asha K Patel^{3

4}, Andrew L Hook¹, Divya Rajamohan³, Vinoj T George³, Luke Flatt³, Minal J Patel³, Vidana C Epa⁵, Taranjit Singh¹, Robert Langer⁴, Daniel G Anderson⁴, Nicholas D Allen⁶, David C Hay⁷, David A Winkler^{8

9

10}, David A Barrett¹¹, Martyn C Davies¹, Lorraine E Young³, Chris Denning³, Morgan R Alexander¹

Affiliations

¹ Laboratory of Biophysics and Surface Analysis, School of Pharmacy, University of Nottingham, Nottingham, NG7 2RD, UK.
² Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, 02115, USA.
³ Wolfson Centre for Stem Cells, Tissue Engineering and Modelling, School of Medicine, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.
⁴ David H. Koch Institute for Integrative Cancer Research, Department of Chemical Engineering, Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
⁵ CSIRO Manufacturing Flagship, 343 Royal Parade, Parkville, 3052, Australia.
⁶ Cardiff School of Biosciences, The Sir Martin Evans Building, Museum Avenue, Cardiff, CF10 3AX, UK.
⁷ MRC Centre for Regenerative Medicine SCRM Building, The University of Edinburgh, Edinburgh BioQuarter, 5 Little France Drive, Edinburgh, EH16 4UU, UK.
⁸ CSIRO Manufacturing Flagship, Bayview Avenue, Clayton, 3168, Australia.
⁹ Monash Institute of Pharmaceutical Sciences, 399 Royal Parade, Parkville, 3052, Australia.
¹⁰ Latrobe Institute for Molecular Science, Latrobe University, Bundoora, 3086, Australia.
¹¹ Centre for Analytical Bioscience, School of Pharmacy, University of Nottingham, Nottingham, NG7 2RD, UK.

Abstract

A scalable and cost-effective synthetic polymer substrate that supports robust expansion and subsequent multilineage differentiation of human pluripotent stem cells (hPSCs) with defined commercial media is presented. This substrate can be applied to common cultureware and used off-the-shelf after long-term storage. Expansion and differentiation of hPSCs are performed entirely on the polymeric surface, enabling the clinical potential of hPSC-derived cells to be realized.

Keywords: high throughput; human pluripotent stem cells; materials discovery; polymer microarrays; stem cell differentiation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Adhesion / physiology
Cell Culture Techniques / instrumentation*
Cell Differentiation / physiology*
Cell Line
Cell Lineage
Culture Media
Fluorescent Antibody Technique
High-Throughput Screening Assays
Humans
Microarray Analysis
Pluripotent Stem Cells / physiology*
Polymers*

Substances

Culture Media
Polymers

Abstract

Publication types

MeSH terms

Substances

Grants and funding