Arenavirus Coinfections Are Common in Snakes with Boid Inclusion Body Disease

J Hepojoki; P Salmenperä; T Sironen; U Hetzel; Y Korzyukov; A Kipar; O Vapalahti

doi:10.1128/JVI.01112-15

Arenavirus Coinfections Are Common in Snakes with Boid Inclusion Body Disease

J Virol. 2015 Aug;89(16):8657-60. doi: 10.1128/JVI.01112-15. Epub 2015 Jun 3.

Authors

J Hepojoki¹, P Salmenperä², T Sironen³, U Hetzel⁴, Y Korzyukov³, A Kipar⁴, O Vapalahti⁵

Affiliations

¹ University of Helsinki, Department of Virology, Haartman Institute, Helsinki, Finland [email protected].
² Blueprint Genetics, Helsinki, Finland.
³ University of Helsinki, Department of Virology, Haartman Institute, Helsinki, Finland.
⁴ Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zürich, Zürich, Switzerland Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland.
⁵ University of Helsinki, Department of Virology, Haartman Institute, Helsinki, Finland Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland Department of Virology and Immunology, University of Helsinki, Hospital District of Helsinki and Uusimaa, Helsinki, Finland.

Abstract

Recently, novel arenaviruses were found in snakes with boid inclusion body disease (BIBD); these form the new genus Reptarenavirus within the family Arenaviridae. We used next-generation sequencing and de novo sequence assembly to investigate reptarenavirus isolates from our previous study. Four of the six isolates and all of the samples from snakes with BIBD contained at least two reptarenavirus species. The viruses sequenced comprise four novel reptarenavirus species and a representative of a new arenavirus genus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Arenaviridae / classification
Arenaviridae / genetics*
Base Sequence
Boidae / virology*
Coinfection / virology*
Genetic Variation*
High-Throughput Nucleotide Sequencing
Inclusion Bodies, Viral / pathology*
Likelihood Functions
Models, Genetic
Molecular Sequence Data
Phylogeny
Reverse Transcriptase Polymerase Chain Reaction
Species Specificity