Genetic and antigenic characterization of Bungowannah virus, a novel pestivirus

P D Kirkland; M J Frost; K R King; D S Finlaison; C L Hornitzky; X Gu; M Richter; I Reimann; M Dauber; H Schirrmeier; M Beer; J F Ridpath

doi:10.1016/j.vetmic.2015.05.014

Genetic and antigenic characterization of Bungowannah virus, a novel pestivirus

Vet Microbiol. 2015 Aug 5;178(3-4):252-9. doi: 10.1016/j.vetmic.2015.05.014. Epub 2015 May 24.

Authors

P D Kirkland¹, M J Frost², K R King³, D S Finlaison⁴, C L Hornitzky⁵, X Gu⁶, M Richter⁷, I Reimann⁸, M Dauber⁹, H Schirrmeier¹⁰, M Beer¹¹, J F Ridpath¹²

Affiliations

¹ Virology Laboratory, Elizabeth Macarthur Agriculture Institute, Woodbridge Rd, Menangle, New South Wales 2568, Australia. Electronic address: [email protected].
² Virology Laboratory, Elizabeth Macarthur Agriculture Institute, Woodbridge Rd, Menangle, New South Wales 2568, Australia. Electronic address: [email protected].
³ Virology Laboratory, Elizabeth Macarthur Agriculture Institute, Woodbridge Rd, Menangle, New South Wales 2568, Australia. Electronic address: [email protected].
⁴ Virology Laboratory, Elizabeth Macarthur Agriculture Institute, Woodbridge Rd, Menangle, New South Wales 2568, Australia. Electronic address: [email protected].
⁵ Virology Laboratory, Elizabeth Macarthur Agriculture Institute, Woodbridge Rd, Menangle, New South Wales 2568, Australia. Electronic address: [email protected].
⁶ Virology Laboratory, Elizabeth Macarthur Agriculture Institute, Woodbridge Rd, Menangle, New South Wales 2568, Australia.
⁷ Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany. Electronic address: [email protected].
⁸ Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany. Electronic address: [email protected].
⁹ Department of Experimental Animal Facilities and Biorisk Management at the Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany. Electronic address: [email protected].
¹⁰ Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany. Electronic address: [email protected].
¹¹ Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany. Electronic address: [email protected].
¹² National Animal Disease Centre, 2300 Dayton Avenue, Ames, IA, 50010, United States. Electronic address: [email protected].

PMID: 26049593
DOI: 10.1016/j.vetmic.2015.05.014

Abstract

Bungowannah virus, a possible new species within the genus Pestivirus, has been associated with a disease syndrome in pigs characterized by myocarditis with a high incidence of stillbirths. The current analysis of the whole-genome and antigenic properties of this virus confirms its unique identity, and further suggests that this virus is both genetically and antigenically remote from previously recognized pestiviruses. There was no evidence of reactivity with monoclonal antibodies (mAbs) that are generally considered to be pan-reactive with other viruses in the genus, and there was little cross reactivity with polyclonal sera. Subsequently, a set of novel mAbs has been generated which allow detection of Bungowannah virus. The combined data provide convincing evidence that Bungowannah virus is a member of the genus Pestivirus and should be officially recognized as a novel virus species.

Keywords: Antigenic characterisation; Bungowannah virus; Characterisation; Full-length genome sequence; Monoclonal antibodies; Pestivirus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Antibodies, Viral / immunology*
Base Sequence
Molecular Sequence Data
Pestivirus / genetics*
Pestivirus / immunology
Pestivirus / isolation & purification
Pestivirus Infections / virology*
Sequence Alignment
Sequence Analysis, DNA / veterinary
Swine
Swine Diseases / virology*

Substances

Antibodies, Viral