c-Myb is required for plasma cell migration to bone marrow after immunization or infection

J Exp Med. 2015 Jun 29;212(7):1001-9. doi: 10.1084/jem.20150191. Epub 2015 Jun 15.

Abstract

Plasma cell migration is crucial to immunity, but little is known about the molecular regulators of their migratory programs. Here, we detail the critical role of the transcription factor c-Myb in determining plasma cell location. In the absence of c-Myb, no IgG(+) antigen-specific plasma cells were detected in the bone marrow after immunization or virus infection. This was correlated with a dramatic reduction of plasma cells in peripheral blood, mislocalization in spleen, and an inability of c-Myb-deficient plasma cells to migrate along a CXCL12 gradient. Therefore, c-Myb plays an essential, novel role in establishing the long-lived plasma cell population in the BM via responsiveness to chemokine migration cues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Bone Marrow / immunology
  • Bone Marrow / metabolism*
  • Cell Movement / physiology*
  • DNA Primers / genetics
  • Enzyme-Linked Immunosorbent Assay
  • Enzyme-Linked Immunospot Assay
  • Flow Cytometry
  • Histological Techniques
  • Immunization*
  • Immunoglobulin G / metabolism
  • Mice
  • Molecular Sequence Data
  • Plasma Cells / metabolism
  • Plasma Cells / physiology*
  • Proto-Oncogene Proteins c-myb / metabolism*
  • Sequence Analysis, DNA
  • Statistics, Nonparametric
  • Virus Diseases / immunology*

Substances

  • DNA Primers
  • Immunoglobulin G
  • Proto-Oncogene Proteins c-myb