Marrying microfluidics and microwells for parallel, high-throughput single-cell genomics

Marc H Wadsworth 2nd; Travis K Hughes; Alex K Shalek

doi:10.1186/s13059-015-0695-0

Marrying microfluidics and microwells for parallel, high-throughput single-cell genomics

Genome Biol. 2015 Jun 20;16(1):129. doi: 10.1186/s13059-015-0695-0.

Authors

Marc H Wadsworth 2nd^{1

2

3

4}, Travis K Hughes^{2

3

4

5}, Alex K Shalek^{6

7

8

9

10

11}

Affiliations

¹ Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, USA.
² Institute for Medical Engineering & Science, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
³ Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA.
⁴ Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, 02115, USA.
⁵ Division of Health Sciences and Technology, Harvard Medical School, Boston, MA, USA.
⁶ Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, USA. [email protected].
⁷ Institute for Medical Engineering & Science, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA. [email protected].
⁸ Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA. [email protected].
⁹ Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, 02115, USA. [email protected].
¹⁰ Division of Health Sciences and Technology, Harvard Medical School, Boston, MA, USA. [email protected].
¹¹ Immunology, MGH, Boston, MA, 02114, USA. [email protected].

Abstract

An innovative, microwell-based platform for single-cell RNA sequencing (RNA-seq) combines cost efficiency, scalability and parallelizability, and will enable many new avenues of biological inquiry.See related Research article: http://dx.doi.org/10.1186/s13059-015-0684-3.

Publication types

Comment

MeSH terms

Gene Expression Profiling / methods*
High-Throughput Nucleotide Sequencing / methods*
Humans
Microfluidic Analytical Techniques / methods*
Sequence Analysis, RNA / methods*