Daucus carota Pentane/Diethyl Ether Fraction Inhibits Motility and Reduces Invasion of Cancer Cells

Chemotherapy. 2014;60(5-6):302-9. doi: 10.1159/000430085. Epub 2015 Jun 13.

Abstract

Daucus carota (DC) is a herb used in folklore medicine in Lebanon to treat numerous diseases including cancer. Recent studies in our laboratory on DC oil and its fractions revealed potent anticancer activities in vitro and in vivo. The present study aims to investigate the effect of the most potent DC fraction, pentane/diethyl ether (50:50), on lung, skin, breast and glioblastoma cancer cell motility and invasion. Upon treatment, a pronounced decrease in cancer cell motility was observed in the 4 cell lines. The treatment also led to a decrease in cancer cell invasion and an increased cell adhesion. Additionally, the DC fraction caused a decrease in the activation of the ρ-GTPases Rac and CDC42, a finding that may partially explain the treatment-induced decrease in cell motility. The current study demonstrates a crucial effect of the DC pentane/diethyl ether fraction on cancer cell motility and metastasis, making it a potential candidate for cancer therapy specifically targeting cancer motility and metastasis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breast Neoplasms / drug therapy
  • Breast Neoplasms / pathology
  • Cell Movement / drug effects*
  • Daucus carota*
  • Ether / isolation & purification
  • Ether / pharmacology
  • Ether / therapeutic use*
  • Female
  • Humans
  • Lung Neoplasms / drug therapy
  • Lung Neoplasms / pathology
  • Melanoma, Experimental
  • Neoplasm Invasiveness / pathology
  • Neoplasm Invasiveness / prevention & control*
  • Pentanes / isolation & purification
  • Pentanes / pharmacology
  • Pentanes / therapeutic use*
  • Plant Extracts / isolation & purification
  • Plant Extracts / therapeutic use*
  • Plant Leaves
  • Plant Oils / isolation & purification
  • Plant Oils / therapeutic use
  • Skin Neoplasms / drug therapy
  • Skin Neoplasms / pathology

Substances

  • Pentanes
  • Plant Extracts
  • Plant Oils
  • Ether
  • pentane