GmHs1-1, encoding a calcineurin-like protein, controls hard-seededness in soybean

Lianjun Sun; Zhenyan Miao; Chunmei Cai; Dajian Zhang; Meixia Zhao; Yanyan Wu; Xueling Zhang; Stephen A Swarm; Liwen Zhou; Zhanyuan J Zhang; Randall L Nelson; Jianxin Ma

doi:10.1038/ng.3339

GmHs1-1, encoding a calcineurin-like protein, controls hard-seededness in soybean

Nat Genet. 2015 Aug;47(8):939-43. doi: 10.1038/ng.3339. Epub 2015 Jun 22.

Authors

Affiliations

¹ Department of Agronomy, Purdue University, West Lafayette, Indiana, USA.
² College of Life Sciences, Qingdao Agricultural University, Qingdao, China.
³ Department of Crop Sciences, University of Illinois, Urbana, Illinois, USA.
⁴ Plant Transformation Core Facility, Division of Plant Sciences, University of Missouri, Columbia, Missouri, USA.
⁵ 1] Department of Crop Sciences, University of Illinois, Urbana, Illinois, USA. [2] Soybean/Maize Germplasm, Pathology, and Genetics Research Unit, US Department of Agriculture-Agricultural Research Service, Urbana, Illinois, USA.
⁶ 1] Department of Agronomy, Purdue University, West Lafayette, Indiana, USA. [2] College of Life Sciences, Qingdao Agricultural University, Qingdao, China.

PMID: 26098868
DOI: 10.1038/ng.3339

Abstract

Loss of seed-coat impermeability was essential in the domestication of many leguminous crops to promote the production of their highly nutritious seeds. Here we show that seed-coat impermeability in wild soybean is controlled by a single gene, GmHs1-1, which encodes a calcineurin-like metallophosphoesterase transmembrane protein. GmHs1-1 is primarily expressed in the Malpighian layer of the seed coat and is associated with calcium content. The transition from impermeability to permeability in domesticated soybean was caused by artificial selection of a point mutation in GmHs1-1. Interestingly, a number of soybean landraces evaded selection for permeability because of an alternative selection for seed-coat cracking that also enables seed imbibition. Despite the single origin of the mutant allele Gmhs1-1, the distribution pattern of allelic variants in the context of soybean population structure and the detected signature of genomic introgression between wild and cultivated soybeans suggest that Gmhs1-1 may have experienced reselection for seed-coat permeability.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Calcineurin / genetics*
Calcineurin / metabolism
Calcium / metabolism
Chromosome Mapping
Chromosomes, Plant / genetics
Gene Expression Regulation, Plant
Genetic Complementation Test
Glycine max / classification
Glycine max / genetics*
Glycine max / metabolism
In Situ Hybridization
Molecular Sequence Data
Mutation
Phylogeny
Plants, Genetically Modified
Promoter Regions, Genetic / genetics
Reverse Transcriptase Polymerase Chain Reaction
Seeds / genetics*
Seeds / metabolism
Sequence Homology, Nucleic Acid
Soybean Proteins / classification
Soybean Proteins / genetics*
Soybean Proteins / metabolism
Species Specificity

Substances

Soybean Proteins
Calcineurin
Calcium

Associated data

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GENBANK/KP698734
GENBANK/KP698735
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GENBANK/KR106134
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