The release of cholecystokinin-8-like (CCK) immunoreactivity from the rat striatum has been studied in vivo using brain microdialysis. A basal efflux of CCK-like immunoreactivity was not detectable in the majority of experiments. Intrastriatal infusion of veratrine (100 micrograms/ml) increased striatal dialysate levels of CCK-like immunoreactivity above detection limits, representing an overflow into the interstitial fluid. High concentrations of potassium caused similar but less consistent effects. Extracellular dopamine depletion using alpha-methyl-p-tyrosine or reserpine also increased the dialysate content of CCK-like immunoreactivity. In contrast, inhibition of peptidases reported to hydrolyse CCK in vitro did not affect either basal or evoked efflux of CCK-like immunoreactivity. These data demonstrate that CCK-like immunoreactivity may be released from neuronal elements within the striatum by depolarizing stimuli in vivo, and suggest that increased overflow of CCK-like immunoreactivity is associated with dopamine depletion.