Relative quantitation of multi-antennary N-glycan classes: combining PGC-LC-ESI-MS with exoglycosidase digestion

J L Abrahams; N H Packer; M P Campbell

doi:10.1039/c5an00691k

Relative quantitation of multi-antennary N-glycan classes: combining PGC-LC-ESI-MS with exoglycosidase digestion

Analyst. 2015 Aug 21;140(16):5444-9. doi: 10.1039/c5an00691k.

Authors

J L Abrahams¹, N H Packer, M P Campbell

Affiliation

¹ Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia. [email protected].

PMID: 26133503
DOI: 10.1039/c5an00691k

Abstract

In the search for N-glycan disease biomarkers current glycoanalytical methods may not be revealing a complete picture of precious samples, and we may be missing valuable structural information that fall outside analysis windows. We report a targeted strategy combining PGC-LC-ESI-MS with exoglycosidases to improve the relative quantitation of tri and tetra-antennary glycan classes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomarkers, Tumor / metabolism*
Chromatography, Liquid / methods*
Glycoside Hydrolases / metabolism*
Humans
Melanoma / diagnosis*
Melanoma / metabolism
Polysaccharides / analysis*
Polysaccharides / metabolism*
Spectrometry, Mass, Electrospray Ionization / methods*
Tandem Mass Spectrometry
Tumor Cells, Cultured

Substances

Biomarkers, Tumor
Polysaccharides
Glycoside Hydrolases