Characterization of Third-Generation-Cephalosporin-Resistant Shiga Toxin-Producing Strains of Escherichia coli O157:H7 in Japan

Ryuji Kawahara; Kazuko Seto; Masumi Taguchi; Chie Nakajima; Yuko Kumeda; Yasuhiko Suzuki

doi:10.1128/JCM.01263-15

Characterization of Third-Generation-Cephalosporin-Resistant Shiga Toxin-Producing Strains of Escherichia coli O157:H7 in Japan

J Clin Microbiol. 2015 Sep;53(9):3035-8. doi: 10.1128/JCM.01263-15. Epub 2015 Jul 1.

Authors

Ryuji Kawahara¹, Kazuko Seto², Masumi Taguchi², Chie Nakajima³, Yuko Kumeda², Yasuhiko Suzuki³

Affiliations

¹ Division of Bacteriology, Osaka Prefectural Institute of Public Health, Osaka, Japan [email protected].
² Division of Bacteriology, Osaka Prefectural Institute of Public Health, Osaka, Japan.
³ Division of Bioresources, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan Hokkaido University, The Global Station for Zoonosis Control, Sapporo, Japan.

Abstract

We isolated Shiga toxin-producing Escherichia coli O157:H7 strains resistant to third-generation cephalosporins. The resistant strains harbored blaCMY-2, a plasmid-mediated AmpC β-lactamase. Genotyping of isolates revealed the possible spread of this problematic bacterium. Results suggested the importance of the investigation and surveillance of enterobacteria with plasmids harboring blaCMY-2.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology*
Cephalosporins / pharmacology*
Escherichia coli Infections / microbiology
Escherichia coli O157 / classification*
Escherichia coli O157 / drug effects*
Escherichia coli O157 / genetics
Escherichia coli O157 / isolation & purification
Genotype
Humans
Japan
Plasmids / analysis
Shiga Toxin / metabolism*
beta-Lactam Resistance*

Substances

Anti-Bacterial Agents
Cephalosporins
Shiga Toxin