Mechanical clearance of red blood cells by the human spleen: Potential therapeutic applications of a biomimetic RBC filtration method

Transfus Clin Biol. 2015 Aug;22(3):151-7. doi: 10.1016/j.tracli.2015.05.004. Epub 2015 Jun 29.

Abstract

During their lifespan, circulating RBC are frequently checked for their deformability. This mechanical quality control operates essentially in the human spleen. RBC unable to squeeze though narrow splenic slits are retained and cleared from the blood circulation. Under physiological conditions this prevents microvessels from being clogged by senescent, rigid RBC. Retention of poorly deformable RBC is an important determinant of pathogenesis in malaria and may also impact the clinical benefit of transfusion. Modulating the splenic retention of RBC has already been proposed to support therapeutic approaches in these research fields. To this aim, the development of microplates for high throughput filtration of RBC through microsphere layers (microplate-based microsphiltration) has been undertaken. This review focuses on potential therapeutic applications provided by this technology in malaria chemotherapy and transfusion.

Keywords: Applications; Applications thérapeutiques; Deformability; Déformabilité; Erythrocyte; Filtration; Malaria; Microspheres; Microsphères; Paludisme; Rate; Spleen; Transfusion; Érythrocyte.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Antimalarials / pharmacology
  • Antimalarials / therapeutic use
  • Biomimetics*
  • Blood Preservation / adverse effects
  • Blood Transfusion
  • Drug Evaluation, Preclinical
  • Equipment Design
  • Erythrocyte Aging
  • Erythrocyte Deformability*
  • Erythrocyte Indices
  • Erythrocytes / drug effects
  • Erythrocytes / parasitology
  • Erythrocytes, Abnormal
  • Filtration / instrumentation
  • Filtration / methods*
  • Humans
  • Malaria / blood
  • Malaria / drug therapy
  • Malaria / therapy
  • Microspheres
  • Parasitemia / blood
  • Parasitemia / drug therapy
  • Parasitemia / therapy
  • Plasmodium / drug effects
  • Plasmodium / growth & development
  • Spleen / physiology*

Substances

  • Antimalarials