Nitrate removal by organotrophic anaerobic ammonium oxidizing bacteria with C2/C3 fatty acid in upflow anaerobic sludge blanket reactors

Yuhai Liang; Dong Li; Xiaojing Zhang; Huiping Zeng; Yin Yang; Jie Zhang

doi:10.1016/j.biortech.2015.06.133

Nitrate removal by organotrophic anaerobic ammonium oxidizing bacteria with C2/C3 fatty acid in upflow anaerobic sludge blanket reactors

Bioresour Technol. 2015 Oct:193:408-14. doi: 10.1016/j.biortech.2015.06.133. Epub 2015 Jul 2.

Authors

Yuhai Liang¹, Dong Li², Xiaojing Zhang³, Huiping Zeng¹, Yin Yang¹, Jie Zhang⁴

Affiliations

¹ Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
² Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China. Electronic address: [email protected].
³ Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, Zhengzhou University of Light Industry, Zhengzhou 450001, China.
⁴ Key Laboratory of Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.

PMID: 26151852
DOI: 10.1016/j.biortech.2015.06.133

Abstract

In anaerobic ammonium oxidation (Anammox) process, a harsh ratio of nitrite to ammonia in influent was demanded, and the max nitrogen removal efficiency could only achieve to 89%, both of which limited the development of Anammox. The aim of this work was to study the nitrate removal by organotrophic anaerobic ammonium oxidizing bacteria (AAOB) with C2/C3 fatty acid in upflow anaerobic sludge blanket (UASB) reactors. In this study, organotrophic AAOB was successfully enriched by adding acetate and propionate with the total organic carbon to nitrogen (TOC/N) ratio of 0.1. In the condition of low substrate, the TN removal efficiency reached 90%, with the effluent TN of around 11.8 mg L(-1). After the addition of acetate and propionate, the predominant species in Anammox granular sludge transformed to Candidatus Jettenia that belonging to organotrophic AAOB from the Candidatus Kuenenia relating to general AAOB.

Keywords: Granular sludge; High-throughput pyrosequencing; Nitrate removal; Organotrophic Anammox.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ammonium Compounds / metabolism*
Bacteria, Anaerobic / metabolism*
Biodegradation, Environmental
Bioreactors / microbiology*
Fatty Acids / metabolism*
High-Throughput Nucleotide Sequencing
Nitrates / isolation & purification*
Nitrogen / isolation & purification
Oxidation-Reduction
Sewage / microbiology*
Wastewater / microbiology
Water Purification

Substances

Ammonium Compounds
Fatty Acids
Nitrates
Sewage
Waste Water
Nitrogen