Compound 331 selectively induces glioma cell death by upregulating miR-494 and downregulating CDC20

Sci Rep. 2015 Jul 8:5:12003. doi: 10.1038/srep12003.

Abstract

Malignant gliomas are the most common malignant tumors in the central nervous system (CNS). Up to date, the prognosis of glioma is still very poor, effective therapy with less side-effect is very necessary. Herein, we identify a compound named as "331" selectively induced cell death in glioma cells but not in astrocytes. Compound 331 upregulated miR-494 and downregulated CDC20 in glioma cells but not in astrocytes. These results suggest that compound 331 could be a potential drug selectively targeting glioma cells through upregulating miR-494 and downregulating CDC20.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects*
  • Astrocytes / cytology
  • Astrocytes / drug effects
  • Cdc20 Proteins / metabolism*
  • Cell Cycle Checkpoints / drug effects
  • Cell Survival / drug effects
  • Cells, Cultured
  • Down-Regulation / drug effects*
  • Female
  • Glioma / drug therapy
  • Glioma / metabolism
  • Glioma / pathology
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • MicroRNAs / metabolism*
  • Pyridines / chemistry
  • Pyridines / pharmacology*
  • Pyridines / therapeutic use
  • Rats
  • Rats, Sprague-Dawley
  • Thiosemicarbazones / chemistry
  • Thiosemicarbazones / pharmacology*
  • Thiosemicarbazones / therapeutic use
  • Transplantation, Heterologous
  • Up-Regulation / drug effects*

Substances

  • Cdc20 Proteins
  • MIRN494 microRNA, human
  • MicroRNAs
  • Pyridines
  • Thiosemicarbazones
  • thiosemicarbazone 331
  • CDC20 protein, human