GSTM1, GSTT1 and GSTP1 Genetic Variants in Multiple Urologic Cancers

Chirurgia (Bucur). 2015 May-Jun;110(3):254-61.

Abstract

Introduction: Glutathione S-transferases (GSTs) are phase 2 enzymes responsible for catalyzing the biotransformation of a wide variety of electrophilic compounds, having a crucial role in the detoxification of active metabolites of procarcinogens produced by phase 1 reactions, tying them to glutathione and promoting their excretion in the urine.

Objectives: we evaluated GSTM1, GSTT1 and GSTP1 genotypes in patients diagnosed with multiple malignancies, of which at least one was found in the prostate, bladder or kidney.

Materials and methods: GSTM1, GSTT1 and GSTP1 genotypes were genetically assessed in 34 patients with multiple urologic cancers and 23 patients with urologic cancer associated with another type of cancer.

Results: in the group of patients with multiple urologic cancers, GSTT1 null genotype was found in 26.4% of patients compared to 0% in controls, 82.35 % of patients and 47% of witnesses carried at least one GSTM1 or GSTT1 null genotype, and in the group with different cancers, GSTM1 null genotype was found in 52.1% of patients compared to 4.3% witnesses in the control group; GSTT1 null genotype was found in 34.7% of patients compared to 4.3% of witnesses, atleast one GSTM1 or GSTT1 null genotype was found in 73.9% of patients compared to 8.6% of controls.

Conclusions: GSTT1 null genotype is a risk factor for patients with more primitive urologic malignancies (bladder, prostate and kidney); GSTM1 or GSTT1 null genotype is more frequent in patients with multiple urologic tumors; GSTM1 and GSTT1 null genotypes are risk factors in patients with different types of cancer, with at least one affecting the urinary system.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor / genetics*
  • Case-Control Studies
  • Female
  • Genotype
  • Glutathione S-Transferase pi / genetics*
  • Glutathione Transferase / genetics*
  • Humans
  • Male
  • Middle Aged
  • Mutation
  • Neoplasms, Multiple Primary / diagnosis
  • Neoplasms, Multiple Primary / enzymology
  • Neoplasms, Multiple Primary / genetics*
  • Neoplasms, Multiple Primary / surgery
  • Polymorphism, Genetic*
  • Predictive Value of Tests
  • Risk Factors
  • Sensitivity and Specificity
  • Urogenital Neoplasms / diagnosis
  • Urogenital Neoplasms / enzymology
  • Urogenital Neoplasms / genetics*
  • Urogenital Neoplasms / surgery
  • Urologic Neoplasms / genetics

Substances

  • Biomarkers, Tumor
  • glutathione S-transferase T1
  • GSTP1 protein, human
  • Glutathione S-Transferase pi
  • Glutathione Transferase
  • glutathione S-transferase M1