Abstract
Chronic myeloid leukemia (CML) is diagnostically defined by the reciprocal translocation t(9;22)(q34;q11). This aberration can be detected by the BCR-ABL fluorescence in situ hybridization (FISH) technique. This article presents a comparative analysis of different commercially available FISH probes and different FISH protocols in order to optimize this technique on formalin-fixed and paraffin-embedded bone marrow trephine biopsies.
Publication types
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Comparative Study
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English Abstract
MeSH terms
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Biopsy, Needle
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Bone Marrow / pathology*
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Chromosomes, Human, Pair 22
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Chromosomes, Human, Pair 9
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Fusion Proteins, bcr-abl / analysis*
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Fusion Proteins, bcr-abl / genetics*
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Humans
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In Situ Hybridization, Fluorescence / instrumentation
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In Situ Hybridization, Fluorescence / methods*
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics*
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / pathology*
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Sensitivity and Specificity
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Translocation, Genetic