Abstract
The delivery of peptide nucleic acids (PNAs) to cells is a very challenging task. We report here that a liposomal formulation composed of egg PC/cholesterol/DSPE-PEG2000 can be loaded, according to different encapsulation techniques, with PNA or fluorescent PNA oligomers. PNA loaded liposomes efficiently and quickly promote the uptake of a PNA targeting the microRNA miR-210 in human erythroleukemic K562 cells. By using this innovative delivery system for PNA, down-regulation of miR-210 is achieved at a low PNA concentration.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Down-Regulation
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Drug Delivery Systems*
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Gene Expression Regulation, Neoplastic
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Humans
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K562 Cells
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Liposomes*
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MicroRNAs / antagonists & inhibitors*
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MicroRNAs / genetics
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Oligonucleotides, Antisense / administration & dosage*
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Peptide Nucleic Acids / administration & dosage*
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Peptide Nucleic Acids / chemistry*
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Peptide Nucleic Acids / pharmacology
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Phosphatidylethanolamines / chemistry
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Polyethylene Glycols / chemistry
Substances
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1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy-poly(ethylene glycol 2000)
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Liposomes
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MIRN210 microRNA, human
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MicroRNAs
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Oligonucleotides, Antisense
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Peptide Nucleic Acids
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Phosphatidylethanolamines
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Polyethylene Glycols