Synthesis and Characterization of pH-Responsive Copolypeptides Vesicles for siRNA and Chemotherapeutic Drug Co-Delivery

Macromol Biosci. 2015 Nov;15(11):1497-506. doi: 10.1002/mabi.201500161. Epub 2015 Jul 17.

Abstract

Novel diblock copolypeptides are synthesized and evaluated as a pH-sensitive drug delivery vector for anticancer drugs and siRNA co-delivery. The synthetic process involve the ring opening polymerization of α-amino acid N-carboxyanhydrides as well as aminolysis and deprotection reactions, and the structures are characterized. The copolypeptides can self-assemble into stable vesicles at neutral pH, and disassemble in acidic endosomal/lysosomal, which shown the pH-responsive behavior. The cell uptake results indicated that the vesicles can co-deliver DOX and siRNA inside the same cell and exhibit a pH-sensitive release behavior. Therefore, the novel polymeric vesicles are a promising carrier for co-delivery anticancer drug and siRNA to overcome the drug resistance and enhance cancer treatment.

Keywords: co-delivery; copolypeptide; pH-responsive; polymersome; vesicles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Drug Carriers / chemical synthesis*
  • Drug Carriers / chemistry*
  • Hydrogen-Ion Concentration
  • Peptides / chemical synthesis*
  • Peptides / chemistry*
  • RNA, Small Interfering / chemistry*

Substances

  • Drug Carriers
  • Peptides
  • RNA, Small Interfering