Potential regulatory phosphorylation sites in a Medicago truncatula plasma membrane proton pump implicated during early symbiotic signaling in roots

Thao T Nguyen; Jeremy D Volkening; Christopher M Rose; Muthusubramanian Venkateshwaran; Michael S Westphall; Joshua J Coon; Jean-Michel Ané; Michael R Sussman

doi:10.1016/j.febslet.2015.06.035

Potential regulatory phosphorylation sites in a Medicago truncatula plasma membrane proton pump implicated during early symbiotic signaling in roots

FEBS Lett. 2015 Aug 4;589(17):2186-93. doi: 10.1016/j.febslet.2015.06.035. Epub 2015 Jul 17.

Authors

Thao T Nguyen¹, Jeremy D Volkening¹, Christopher M Rose², Muthusubramanian Venkateshwaran³, Michael S Westphall², Joshua J Coon⁴, Jean-Michel Ané⁵, Michael R Sussman⁶

Affiliations

¹ Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, United States; Biotechnology Center, University of Wisconsin-Madison, Madison, WI 53706, United States.
² Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, United States; Genome Center of Wisconsin, University of Wisconsin-Madison, Madison, WI 53706, United States.
³ Department of Agronomy, University of Wisconsin-Madison, Madison, WI 53706, United States; School of Agriculture, University of Wisconsin-Platteville, Platteville, WI 53818, United States.
⁴ Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, United States; Genome Center of Wisconsin, University of Wisconsin-Madison, Madison, WI 53706, United States; Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, WI 53706, United States.
⁵ Department of Agronomy, University of Wisconsin-Madison, Madison, WI 53706, United States.
⁶ Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, United States; Biotechnology Center, University of Wisconsin-Madison, Madison, WI 53706, United States. Electronic address: [email protected].

Abstract

In plants and fungi the plasma membrane proton pump generates a large proton-motive force that performs essential functions in many processes, including solute transport and the control of cell elongation. Previous studies in yeast and higher plants have indicated that phosphorylation of an auto-inhibitory domain is involved in regulating pump activity. In this report we examine the Medicago truncatula plasma membrane proton pump gene family, and in particular MtAHA5. Yeast complementation assays with phosphomimetic mutations at six candidate sites support a phosphoregulatory role for two residues, suggesting a molecular model to explain early Nod factor-induced changes in the plasma membrane proton-motive force of legume root cells.

Keywords: Medicago truncatula; Nod factor; PM H(+)-ATPase; Phosphoproteomics; Phosphorylation; Proton pump.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Amino Acid Sequence
Binding Sites / genetics
Blotting, Western
Cell Membrane / enzymology*
Cluster Analysis
Gene Expression Regulation, Enzymologic
Genetic Complementation Test
Host-Pathogen Interactions
Medicago truncatula / enzymology*
Medicago truncatula / genetics
Medicago truncatula / microbiology
Molecular Sequence Data
Multigene Family
Mutation
Phosphorylation
Phylogeny
Plant Proteins / classification
Plant Proteins / genetics
Plant Proteins / metabolism*
Plant Roots / enzymology*
Plant Roots / genetics
Plant Roots / microbiology
Proton-Translocating ATPases / classification
Proton-Translocating ATPases / genetics
Proton-Translocating ATPases / metabolism*
Rhizobium / physiology
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / growth & development
Sequence Homology, Amino Acid
Serine / genetics
Serine / metabolism
Signal Transduction
Symbiosis
Threonine / genetics
Threonine / metabolism

Substances

Plant Proteins
Threonine
Serine
Proton-Translocating ATPases

Abstract

Publication types

MeSH terms

Substances

Grants and funding