Abstract
We report the genome sequence of Planobispora rosea ATCC 53733, a mycelium-forming soil-dweller belonging to one of the lesser studied genera of Actinobacteria and producing the thiopeptide GE2270. The P. rosea genome presents considerable convergence in gene organization and function with other members in the family Streptosporangiaceae, with a significant number (44%) of shared orthologs. Patterns of gene expression in P. rosea cultures during exponential and stationary phase have been analyzed using whole transcriptome shotgun sequencing and by proteome analysis. Among the differentially abundant proteins, those involved in protein metabolism are particularly represented, including the GE2270-insensitive EF-Tu. Two proteins from the pbt cluster, directing GE2270 biosynthesis, slightly increase their abundance values over time. While GE2270 production starts during the exponential phase, most pbt genes, as analyzed by qRT-PCR, are down-regulated. The exception is represented by pbtA, encoding the precursor peptide of the ribosomally synthesized GE2270, whose expression reached the highest level at the entry into stationary phase.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actinomycetales / genetics*
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Actinomycetales / growth & development
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Actinomycetales / metabolism*
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Bacterial Proteins / analysis
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Bacterial Proteins / metabolism
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Genome, Bacterial*
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Genomics
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Glucose / metabolism
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High-Throughput Nucleotide Sequencing
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Metabolic Networks and Pathways / genetics
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Multigene Family
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Peptides, Cyclic / biosynthesis*
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Proteome / analysis*
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RNA, Bacterial / analysis
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Sequence Analysis, RNA
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Thiazoles
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Transcriptome*
Substances
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Bacterial Proteins
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Peptides, Cyclic
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Proteome
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RNA, Bacterial
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Thiazoles
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Glucose
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GE 2270 A
Grants and funding
This work was supported by Regione Lombardia (ID project 13810096) and by Ministero dell’Istruzione, dell’Università e della Ricerca (MIUR) (project). J.C. was supported by funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement N° 289285. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The funders provided support in the form of salaries for authors [AT, JC, SD and MS], but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the ‘author contributions’ section.