Nitrogen-removal efficiency of a novel aerobic denitrifying bacterium, Pseudomonas stutzeri strain ZF31, isolated from a drinking-water reservoir

Bioresour Technol. 2015 Nov:196:209-16. doi: 10.1016/j.biortech.2015.07.059. Epub 2015 Jul 29.

Abstract

An aerobic denitrifier, identified as Pseudomonas stutzeri strain ZF31, was isolated from the Zhoucun drinking-water reservoir. Strain ZF31 removed 97% of nitrate nitrogen after 16h, without nitrite accumulation. Sequence amplification indicated the presence of the denitrification genes napA, nirS, norB, and nosZ. Nitrogen balance analysis revealed that approximately 75% of the initial nitrogen was removed as gas products. Response surface methodology (RSM) experiments showed that maximum removal of total nitrogen (TN) occurred at pH 8.23, a C/N ratio of 6.68, temperature of 27.72°C, and with shaking at 54.15rpm. The TN removal rate at low C/N ratio (i.e., 3) and low temperature (i.e., 10°C) was 73.30% and 60.08%, respectively. These results suggest that strain ZF31 has potential applications for the bioremediation of slightly polluted drinking-water reservoirs.

Keywords: Aerobic denitrifier; Nitrogen removal; Pseudomonas stutzeri; Reservoir; Response surface methodology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aerobiosis
  • Biodegradation, Environmental
  • Denitrification*
  • Drinking Water / microbiology
  • Models, Chemical
  • Nitrates / analysis
  • Nitrates / metabolism*
  • Nitrites / analysis
  • Nitrogen / metabolism*
  • Pseudomonas stutzeri / enzymology*
  • Pseudomonas stutzeri / genetics
  • Pseudomonas stutzeri / isolation & purification

Substances

  • Drinking Water
  • Nitrates
  • Nitrites
  • Nitrogen