Symbiotic effectiveness and phylogeny of rhizobia isolated from faba bean (Vicia faba L.) in Sichuan hilly areas, China

Kai Wei Xu; Lan Zou; Petri Penttinen; Ke Wang; Nan Nan Heng; Xiao Ping Zhang; Qiang Chen; Ke Zhao; Yuan Xue Chen

doi:10.1016/j.syapm.2015.06.009

Symbiotic effectiveness and phylogeny of rhizobia isolated from faba bean (Vicia faba L.) in Sichuan hilly areas, China

Syst Appl Microbiol. 2015 Oct;38(7):515-23. doi: 10.1016/j.syapm.2015.06.009. Epub 2015 Jul 22.

Authors

Kai Wei Xu¹, Lan Zou², Petri Penttinen³, Ke Wang⁴, Nan Nan Heng⁵, Xiao Ping Zhang⁶, Qiang Chen⁷, Ke Zhao⁸, Yuan Xue Chen⁹

Affiliations

¹ Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: [email protected].
² Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: [email protected].
³ Department of Environmental Sciences, University of Helsinki, Helsinki, Fin-00014, Finland. Electronic address: [email protected].
⁴ Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: [email protected].
⁵ Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: [email protected].
⁶ Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: [email protected].
⁷ Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: [email protected].
⁸ Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: [email protected].
⁹ Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address: [email protected].

PMID: 26242694
DOI: 10.1016/j.syapm.2015.06.009

Abstract

A total of 54 rhizobial strains were isolated from faba bean root nodules in 21 counties of Sichuan hilly areas in China, and their symbiotic effectiveness, genetic diversity and phylogeny were assessed. Only six strains increased the shoot dry mass of the host plant significantly (P ≤ 0.05). Based on the cluster analysis of combined 16S rDNA and intergenic spacer region (IGS) PCR-RFLP, the strains were divided into 31 genotypes in 11 groups, indicating a high degree of genetic diversity among the strains. The sequence analysis of three housekeeping genes (atpD, glnII and recA) and 16S rDNA indicated that the strains represented two R. leguminosarum, two Rhizobium spp., R. mesosinicum, Agrobacterium sp. and A. tumefaciens. The strains representing four Rhizobium species were divided into two distinct nodC and nifH genotypes. However, the phylogeny of housekeeping genes and symbiotic genes was not congruent, implying that the strains had been shaped by vertical evolution of the housekeeping genes and lateral evolution of the symbiotic genes.

Keywords: Faba bean ;·Symbiotic effectiveness ;·Genetic diversity ;·MLSA; Rhizobia.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agrobacterium tumefaciens
China
Cluster Analysis
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
DNA, Ribosomal Spacer / chemistry
DNA, Ribosomal Spacer / genetics
Genetic Variation*
Phylogeny*
Plant Development
RNA, Ribosomal, 16S / genetics
Rhizobiaceae / classification*
Rhizobiaceae / genetics
Rhizobiaceae / isolation & purification*
Rhizobiaceae / physiology
Root Nodules, Plant / microbiology*
Sequence Analysis, DNA
Symbiosis*
Vicia faba / microbiology*

Substances

DNA, Bacterial
DNA, Ribosomal
DNA, Ribosomal Spacer
RNA, Ribosomal, 16S