Cloning, expression, purification and crystallographic studies of galectin-11 from domestic sheep (Ovis aries)

Acta Crystallogr F Struct Biol Commun. 2015 Aug;71(Pt 8):993-7. doi: 10.1107/S2053230X15010195. Epub 2015 Jul 28.

Abstract

Galectins are an evolutionarily conserved family of proteins that translate glycan recognition into cellular effects. Galectin-11 is a unique member of the galectin family that is only expressed in ruminants such as sheep, goat and cattle and that plays a critical role in several important biological processes, such as reproduction and parasite-mediated innate immune responses. Currently, these two areas are of major importance for the sustainability of ruminant livestock production. Despite the emerging biological significance of galectin-11, no structural information is available. It is expected that structural studies will unravel the functional mechanisms of galectin-11 activity. Here, the expression, purification and crystallization of the ruminant-specific galectin-11 from domestic sheep and the collection of X-ray data to 2.0 Å resolution are reported.

Keywords: galectin; glycan; livestock; parasite.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • Crystallization
  • Crystallography, X-Ray
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Galectins / chemistry*
  • Galectins / genetics
  • Gene Expression
  • Molecular Sequence Data
  • Recombinant Fusion Proteins / chemistry*
  • Recombinant Fusion Proteins / genetics
  • Sequence Alignment
  • Sheep, Domestic

Substances

  • Galectins
  • Recombinant Fusion Proteins