Abstract
An FAD-dependent glucose dehydrogenase (GDH) from Aspergillus terreus was purified and crystallized at 293 K using the sitting-drop vapour-diffusion method. A data set was collected to a resolution of 1.6 Å from a single crystal at 100 K using a rotating-anode X-ray source. The crystal belonged to space group P21, with unit-cell parameters a = 56.56, b = 135.74, c = 74.13 Å, β = 90.37°. The asymmetric unit contained two molecules of GDH. The Matthews coefficient was calculated to be 2.2 Å(3) Da(-1) and the solvent content was estimated to be 44%.
Keywords:
FAD; glucose dehydrogenase; glucose sensor.
MeSH terms
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Amino Acid Sequence
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Aspergillus / chemistry*
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Aspergillus / enzymology
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Aspergillus oryzae / genetics
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Aspergillus oryzae / metabolism
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Cloning, Molecular
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Crystallization
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Crystallography, X-Ray
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Flavin-Adenine Dinucleotide / chemistry*
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Fungal Proteins / chemistry*
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Fungal Proteins / genetics
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Gene Expression
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Glucose 1-Dehydrogenase / chemistry*
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Glucose 1-Dehydrogenase / genetics
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Molecular Sequence Data
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Plasmids / chemistry
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Plasmids / metabolism
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Protein Multimerization
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Sequence Alignment
Substances
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Fungal Proteins
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Recombinant Proteins
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Flavin-Adenine Dinucleotide
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Glucose 1-Dehydrogenase