Abstract
Rotaviruses (RVs) are an important cause of severe gastroenteritis in children. It has been found that RV may recognize the histo-blood group antigens (HBGAs) as ligands or receptors and bind HBGAs in a type-dependent manner. In this study, we investigated the binding specificity of VP8* proteins from human rotaviruses (RV) that are prevalent in China including genotypes P[4], P[6], and P[8]. Through the saliva- and oligosaccharide-based binding assays, we found that the VP8* proteins of P[4] and P[8] RV showed similar reactivity with the Leb and H type 1 antigens, while P[6] RV weakly bound the Leb antigen. These findings may facilitate further research into RV host specificity and vaccine development.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Animals
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Antigens, Viral / genetics
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Antigens, Viral / metabolism*
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Blood Group Antigens / genetics
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Blood Group Antigens / metabolism*
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Child
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Female
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Gastroenteritis / blood
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Gastroenteritis / virology
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Genotype
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Humans
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Molecular Sequence Data
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Protein Binding
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RNA-Binding Proteins / metabolism
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Rabbits
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Rotavirus / genetics*
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Rotavirus / immunology
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Rotavirus / metabolism*
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Rotavirus Infections / blood
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Saliva / metabolism
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Saliva / virology
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Viral Nonstructural Proteins / metabolism
Substances
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Antigens, Viral
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Blood Group Antigens
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RNA-Binding Proteins
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Viral Nonstructural Proteins
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NS35 protein, rotavirus
Associated data
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GENBANK/KT162984
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GENBANK/KT162985
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GENBANK/KT162986
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GENBANK/KT162987
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GENBANK/KT162988
Grants and funding
The research was supported by the National Nature Science Foundation of China (NSFC Grant no. 81472003). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.