Protein A-Based Immunoadsorption Is More Efficient Than Conventional Plasma Exchange to Remove Circulating Anti-HLA Antibodies

Blood Purif. 2015;40(2):167-72. doi: 10.1159/000437041.

Abstract

We retrospectively evaluated the ability of protein-A immunoadsorption (IA) as compared to that of conventional plasma exchanges (PE) in reducing the mean fluorescence intensity (MFI) of anti-HLA antibodies assessed by the single antigen assay in sensitized renal transplant recipients. Change in MFI of 441 anti-HLA antibodies was measured after 1 single session of IA or after 3 consecutive daily PE sessions. While both strategies were able to significantly lower the amount of anti-HLA antibodies, the relative reduction in MFI was higher after IA as compared to PE (-69 vs. -58%, respectively, p = 0.003). This better efficacy of IA was observed despite a lower total volume of treated plasma (105 ± 6 vs. 160 ± 16 ml/kg after IA and after PE, respectively). Our data suggest a higher efficiency of IA over conventional PE sessions to remove anti-HLA antibodies and call for a larger evaluation of IA to confirm its potential added value in desensitization protocols.

MeSH terms

  • Antibodies / blood
  • Antibodies / isolation & purification*
  • HLA Antigens / blood
  • HLA Antigens / immunology
  • Humans
  • Immunosorbent Techniques*
  • Kidney Transplantation
  • Plasma Exchange / instrumentation
  • Plasma Exchange / methods*
  • Renal Insufficiency, Chronic / immunology
  • Renal Insufficiency, Chronic / pathology
  • Renal Insufficiency, Chronic / surgery
  • Renal Insufficiency, Chronic / therapy*
  • Retrospective Studies
  • Staphylococcal Protein A / chemistry*

Substances

  • Antibodies
  • HLA Antigens
  • Staphylococcal Protein A