Abstract
Background/aims:
MicroRNAs (miRNAs) have a key role in carcinogenesis and cancer development, but the role of miRNAs in the progression of esophageal cancer (EC) remains unclear.
Methods:
The TE-1 and Eca-109 EC cell lines were used. The expression of miR-491 and candidate gene TPX2 in EC samples (n=99) were detected by RT-PCR. The cells invasive ability was determined by transwell assay. The luciferase reporter assay was used to confirm the regulation mechanism.
Results:
A decreased expression of miR-491 was detected in the EC clinical samples compared with the corresponding adjacent tumor tissues. Aberrant expression of miR-491 regulated cells invasion and EMT markers. Furthermore, we verified that TPX2 was a target gene of miR-491.
Conclusions:
miR-491 may play a critical role in EC.
© 2015 S. Karger AG, Basel.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aged
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Base Sequence
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Cell Cycle Proteins / genetics*
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Cell Cycle Proteins / metabolism
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Cell Line, Tumor
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Cell Proliferation
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Down-Regulation / genetics
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Epithelial-Mesenchymal Transition / genetics
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Esophageal Neoplasms / genetics*
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Esophageal Neoplasms / pathology*
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Female
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Gene Expression Regulation, Neoplastic*
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Gene Knockdown Techniques
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Humans
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Male
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MicroRNAs / genetics
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MicroRNAs / metabolism
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Microtubule-Associated Proteins / genetics*
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Microtubule-Associated Proteins / metabolism
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Middle Aged
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Molecular Sequence Data
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Neoplasm Invasiveness
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Nuclear Proteins / genetics*
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Nuclear Proteins / metabolism
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RNA, Small Interfering / metabolism
Substances
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Cell Cycle Proteins
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MIRN491 microRNA, human
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MicroRNAs
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Microtubule-Associated Proteins
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Nuclear Proteins
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RNA, Small Interfering
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TPX2 protein, human