A 4-week study of four 3-monochloropropane-1,2-diol diesters on lipid metabolism in C57BL/6J mice

Environ Toxicol Pharmacol. 2015 Sep;40(2):453-8. doi: 10.1016/j.etap.2015.07.017. Epub 2015 Jul 28.

Abstract

3-Monochloropropane-1,2-diol (3-MCPD) esters have been detected in many foods, which have become a new safety issue worldwide. In the study, we investigated the effect of four 3-MCPD diesters (palmitate diester: CDP; stearate diester: CDS; oleate diester: CDO; linoleate diester: CDL) on lipid metabolism in C57BL/6J mice. The results showed that CDP, CDS, CDO and CDL significantly increased the serum TC, LDL-C levels and liver TG, TC levels at dose of 16.5μmol/kg/day. These results indicated that 3-MCPD diesters could potentially cause hyperlipidemia in C57BL/6J mice. Moreover, oil red O staining confirmed fat accumulation in liver induced by 3-MCPD diesters. Our work will provide more information for safety evaluation of 3-MCPD diesters. However, whether free 3-MCPD or free fatty acids or combined action compensates for the hyperlipidemia effects should be elucidated in the future.

Keywords: 3-MCPD diesters; C57BL/6J mice; Hyperlipidemia; In vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers / blood*
  • Body Weight / drug effects
  • Dose-Response Relationship, Drug
  • Hyperlipidemias / etiology
  • Linoleic Acid / administration & dosage
  • Linoleic Acid / pharmacology
  • Lipid Metabolism / drug effects*
  • Lipids / blood*
  • Liver / metabolism*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Oleic Acid / administration & dosage
  • Oleic Acid / pharmacology
  • Palmitates / administration & dosage
  • Palmitates / pharmacology
  • alpha-Chlorohydrin / administration & dosage*
  • alpha-Chlorohydrin / pharmacology

Substances

  • Biomarkers
  • Lipids
  • Palmitates
  • Oleic Acid
  • alpha-Chlorohydrin
  • Linoleic Acid