Increasing DNA reactivity and in vitro antitumor activity of trans diiodido Pt(II) complexes with UVA light

J Inorg Biochem. 2015 Dec:153:211-218. doi: 10.1016/j.jinorgbio.2015.07.008. Epub 2015 Jul 21.

Abstract

Trans diiodido platinum(II) complexes bearing the same as well as different aliphatic amines (mixed-amines) have interesting biological activity; cytotoxicity and interactions with some important biological models have already been demonstrated. Herein we described the interaction of such compounds with ct-DNA, supercoiled and linearized plasmid DNA and 5-GMP. Interestingly, UV irradiation of these compounds results in an increase in reactivity towards DNA and 5-GMP in such model systems. Additionally, the cytotoxicity of the trans-Pt(II) complexes towards human cancer cells is noticeably increased when treatment is combined for 90min with UVA-irradiation. With this work we provide evidence that trans diiodido compounds can be activated by UV-light over relatively short treatment times.

Keywords: DNA interactions; Metallodrug photoactivaton; Platinum drugs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / radiation effects*
  • Antineoplastic Agents / toxicity
  • Cell Line, Tumor
  • DNA / chemistry*
  • Humans
  • Iodides / chemistry
  • Organoplatinum Compounds / chemistry
  • Organoplatinum Compounds / radiation effects*
  • Organoplatinum Compounds / toxicity
  • Ultraviolet Rays*

Substances

  • Antineoplastic Agents
  • Iodides
  • Organoplatinum Compounds
  • DNA