Abstract
An accurate dissection of sources of cell-to-cell variability is crucial for quantitative biology at the single-cell level but has been challenging for the cell cycle. We present Cycler, a robust method that constructs a continuous trajectory of cell-cycle progression from images of fixed cells. Cycler handles heterogeneous microenvironments and does not require perturbations or genetic markers, making it generally applicable to quantifying multiple sources of cell-to-cell variability in mammalian cells.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Cell Cycle* / genetics
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Cell Proliferation
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Cyclin A / metabolism
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DNA Replication
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Glycogen Synthase Kinase 3 / metabolism
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HeLa Cells
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Humans
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Image Processing, Computer-Assisted / methods*
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Proliferating Cell Nuclear Antigen / metabolism
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Reproducibility of Results
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Single-Cell Analysis / methods*
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Support Vector Machine
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Tubulin / metabolism
Substances
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Cyclin A
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Proliferating Cell Nuclear Antigen
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Tubulin
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Glycogen Synthase Kinase 3