Raman microscopy as a novel tool to detect endothelial dysfunction

Malgorzata Baranska; Agnieszka Kaczor; Kamilla Malek; Aleksandra Jaworska; Katarzyna Majzner; Emilia Staniszewska-Slezak; Marta Z Pacia; Grzegorz Zajac; Jakub Dybas; Ewelina Wiercigroch

doi:10.1016/j.pharep.2015.03.015

Raman microscopy as a novel tool to detect endothelial dysfunction

Pharmacol Rep. 2015 Aug;67(4):736-43. doi: 10.1016/j.pharep.2015.03.015. Epub 2015 Apr 13.

Affiliations

¹ Faculty of Chemistry, Jagiellonian University, Kraków, Poland; Jagiellonian Center for Experimental Therapeutics (JCET), Jagiellonian University, Kraków, Poland. Electronic address: [email protected].
² Faculty of Chemistry, Jagiellonian University, Kraków, Poland; Jagiellonian Center for Experimental Therapeutics (JCET), Jagiellonian University, Kraków, Poland.
³ Jagiellonian Center for Experimental Therapeutics (JCET), Jagiellonian University, Kraków, Poland.

PMID: 26321275
DOI: 10.1016/j.pharep.2015.03.015

Abstract

Raman microscopy, a label-free method with high spatial resolution, shows growing potential in various fields of medical diagnostics. Several proof-of-concept studies related to the application of Raman microscopy to detect endothelial dysfunction are summarized in this work. Both ex vivo measurements of the tissues in the murine models of endothelial pathologies, as well as in vitro investigations of the cell cultures in the context of cellular transport, drug action and inflammation processes are discussed. The future directions in application of Raman spectroscopy-based methods in such studies are also described.

Keywords: Diabetes; Raman imaging; SERS; Single cell analysis; Systemic hypertension.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Endothelium, Vascular / metabolism
Endothelium, Vascular / pathology*
Humans
Microscopy, Confocal / methods*
Spectrum Analysis, Raman / methods*
Vascular Diseases / diagnosis
Vascular Diseases / metabolism