Impact of Phosphorus-Based Food Additives on Bone and Mineral Metabolism

J Clin Endocrinol Metab. 2015 Nov;100(11):4264-71. doi: 10.1210/jc.2015-2279. Epub 2015 Aug 31.

Abstract

Context: Phosphorus-based food additives can substantially increase total phosphorus intake per day, but the effect of these additives on endocrine factors regulating bone and mineral metabolism is unclear.

Objective: This study aimed to examine the effect of phosphorus additives on markers of bone and mineral metabolism. Design and Setting, and Participants: This was a feeding study of 10 healthy individuals fed a diet providing ∼1000 mg of phosphorus/d using foods known to be free of phosphorus additives for 1 week (low-additive diet), immediately followed by a diet containing identical food items; however, the foods contained phosphorus additives (additive-enhanced diet). Parallel studies were conducted in animals fed low- (0.2%) and high- (1.8%) phosphorus diets for 5 or 15 weeks.

Main outcome measures: The changes in markers of mineral metabolism after each diet period were measured.

Results: Participants were 32 ± 8 years old, 30% male, and 70% black. The measured phosphorus content of the additive-enhanced diet was 606 ± 125 mg higher than the low-additive diet (P < .001). After 1 week of the low-additive diet, consuming the additive-enhanced diet for 1 week significantly increased circulating fibroblast growth factor 23 (FGF23), osteopontin, and osteocalcin concentrations by 23, 10, and 11%, respectively, and decreased mean sclerostin concentrations (P < .05 for all). Similarly, high-phosphorus diets in mice significantly increased blood FGF23, osteopontin and osteocalcin, lowered sclerostin, and decreased bone mineral density (P < .05 for all).

Conclusions: The enhanced phosphorus content of processed foods can disturb bone and mineral metabolism in humans. The results of the animal studies suggest that this may compromise bone health.

Trial registration: ClinicalTrials.gov NCT01394146.

Publication types

  • Clinical Trial
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Adult
  • Animals
  • Biomarkers / metabolism
  • Bone Density / drug effects
  • Bone Morphogenetic Proteins / blood
  • Bone and Bones / drug effects
  • Bone and Bones / metabolism*
  • Diet
  • Feeding Behavior
  • Female
  • Fibroblast Growth Factor-23
  • Fibroblast Growth Factors / blood
  • Food Additives / pharmacology*
  • Genetic Markers
  • Humans
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Middle Aged
  • Minerals / metabolism*
  • Osteocalcin / blood
  • Osteopontin / blood
  • Phosphorus Compounds / pharmacology*
  • Young Adult

Substances

  • Adaptor Proteins, Signal Transducing
  • Biomarkers
  • Bone Morphogenetic Proteins
  • FGF23 protein, human
  • Fgf23 protein, mouse
  • Food Additives
  • Genetic Markers
  • Minerals
  • Phosphorus Compounds
  • SOST protein, human
  • Osteocalcin
  • Osteopontin
  • Fibroblast Growth Factors
  • Fibroblast Growth Factor-23

Associated data

  • ClinicalTrials.gov/NCT01394146