Lead screening in DBS by solid sampling high-resolution continuum source graphite furnace atomic absorption spectrometry: application to newborns and pregnant women

Bioanalysis. 2015;7(16):2057-70. doi: 10.4155/bio.15.124.

Abstract

Background: DBS have become a clinical specimen especially adequate for establishing home-based collection protocols. In this work, high-resolution continuum source graphite furnace atomic absorption spectrometry is evaluated for the direct monitoring of Pb in DBS, both as a quantitative tool and a screening method.

Methodology: The development of the screening model is based on the establishment of the unreliability region around the threshold limits, 100 or 50 μg l(-1). More than 500 samples were analyzed to validate the model.

Conclusion: The screening method demonstrated high sensitivity (the rate of true positives detected was always higher than 95%), an excellent LOD (1 µg l(-1)) and high throughput (10 min per sample).

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Calibration
  • Dried Blood Spot Testing / methods*
  • Female
  • Graphite
  • High-Throughput Screening Assays / methods
  • Humans
  • Infant, Newborn
  • Lead / blood*
  • Limit of Detection
  • Mass Screening / methods
  • Neonatal Screening / methods
  • Pregnancy
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrophotometry, Atomic / instrumentation
  • Spectrophotometry, Atomic / methods*

Substances

  • Lead
  • Graphite