Preclinical assessment of the interactions between the antiretroviral drugs, ritonavir and efavirenz, and the tyrosine kinase inhibitor erlotinib

Cancer Chemother Pharmacol. 2015 Oct;76(4):813-9. doi: 10.1007/s00280-015-2856-y. Epub 2015 Sep 2.

Abstract

Purpose: Prevalence of non-AIDS-defining cancers (NADCs) has increased in the era of potent antiretroviral treatments. Incidence rates of NADCs now exceed AIDS-defining cancers in HIV-positive patients. Treatment of NADCs may be complicated by interactions between antiretrovirals and chemotherapy mostly via inhibition or induction of CYP3A4. Erlotinib is used to treat non-small cell lung and pancreatic cancer and is primarily metabolized by CYP3A4 into multiple products including the active metabolite (OSI-420). Preclinical in vivo assessment was performed to gain a better understanding of CYP3A4-mediated interactions between antiretrovirals and erlotinib.

Methods: Erlotinib (50 mg/kg p.o.) was administered to male FVB mice in the presence and absence of dexamethasone (10 mg/kg p.o. QDx4), efavirenz (25 mg/kg p.o. QDx4), ketoconazole (50 mg/kg p.o.), or ritonavir (12.5 mg/kg p.o.). Blood samples were collected to characterize exposure (AUC).

Results: Administration of erlotinib with CYP3A4 inducers (dexamethasone) and inhibitors (ketoconazole and ritonavir) resulted in significant alterations in erlotinib exposure. Ketoconazole and ritonavir resulted in a 1.7- and 3.0-fold increase in erlotinib AUC, respectively, while dexamethasone results in a 0.6-fold decrease in erlotinib AUC. The CYP3A4 inducer efavirenz did not have a significant effect on erlotinib exposure.

Conclusion: CYP3A4 inducers and inhibitors altered the exposure of erlotinib. Until a definitive clinical trial is performed, erlotinib should be used with caution in patients on a ritonavir-containing antiretroviral regimen, while standard doses may be appropriate for patients on an efavirenz-containing antiretroviral regimen.

Keywords: AIDS; Drug interaction; Efavirenz; Erlotinib; Ritonavir.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Administration, Oral
  • Alkynes
  • Animals
  • Anti-Retroviral Agents / administration & dosage
  • Anti-Retroviral Agents / pharmacology*
  • Antineoplastic Agents / administration & dosage
  • Antineoplastic Agents / blood
  • Antineoplastic Agents / pharmacokinetics*
  • Benzoxazines / administration & dosage
  • Benzoxazines / pharmacology
  • Biological Availability
  • Biotransformation / drug effects
  • Cyclopropanes
  • Cytochrome P-450 CYP3A Inducers / administration & dosage
  • Cytochrome P-450 CYP3A Inducers / pharmacology*
  • Cytochrome P-450 CYP3A Inhibitors / administration & dosage
  • Cytochrome P-450 CYP3A Inhibitors / pharmacology*
  • Dexamethasone / administration & dosage
  • Dexamethasone / pharmacology
  • Drug Evaluation, Preclinical
  • Drug Interactions
  • Erlotinib Hydrochloride / administration & dosage
  • Erlotinib Hydrochloride / blood
  • Erlotinib Hydrochloride / pharmacokinetics*
  • Half-Life
  • Ketoconazole / administration & dosage
  • Ketoconazole / pharmacology
  • Male
  • Metabolic Clearance Rate / drug effects
  • Mice, Inbred Strains
  • Protein Kinase Inhibitors / administration & dosage
  • Protein Kinase Inhibitors / blood
  • Protein Kinase Inhibitors / pharmacokinetics*
  • Quinazolines / blood
  • Ritonavir / administration & dosage
  • Ritonavir / pharmacology*

Substances

  • Alkynes
  • Anti-Retroviral Agents
  • Antineoplastic Agents
  • Benzoxazines
  • Cyclopropanes
  • Cytochrome P-450 CYP3A Inducers
  • Cytochrome P-450 CYP3A Inhibitors
  • OSI-420
  • Protein Kinase Inhibitors
  • Quinazolines
  • Dexamethasone
  • Erlotinib Hydrochloride
  • efavirenz
  • Ritonavir
  • Ketoconazole