Enhancing Quantum Dots for Bioimaging using Advanced Surface Chemistry and Advanced Optical Microscopy: Application to Silicon Quantum Dots (SiQDs)

Xiaoyu Cheng; Elizabeth Hinde; Dylan M Owen; Stuart B Lowe; Peter J Reece; Katharina Gaus; J Justin Gooding

doi:10.1002/adma.201503223

Enhancing Quantum Dots for Bioimaging using Advanced Surface Chemistry and Advanced Optical Microscopy: Application to Silicon Quantum Dots (SiQDs)

Adv Mater. 2015 Oct 28;27(40):6144-50. doi: 10.1002/adma.201503223. Epub 2015 Aug 31.

Authors

Xiaoyu Cheng¹, Elizabeth Hinde², Dylan M Owen², Stuart B Lowe¹, Peter J Reece³, Katharina Gaus², J Justin Gooding¹

Affiliations

¹ School of Chemistry, Australian Centre for NanoMedicine, ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, The University of New South Wales, Sydney, NSW, 2052, Australia.
² EMBL Australia Node in Single Molecule Science, School of Medical Sciences, ARC Centre of Excellence in Advanced Molecular Imaging, The University of New South Wales, Sydney, NSW, 2052, Australia.
³ School of Physics, The University of New South Wales, Sydney, NSW, 2052, Australia.

PMID: 26331712
DOI: 10.1002/adma.201503223

Abstract

Fluorescence lifetime imaging microscopy is successfully demonstrated in both one- and two-photon cases with surface modified, nanocrystalline silicon quantum dots in the context of bioimaging. The technique is further demonstrated in combination with Förster resonance energy transfer studies where the color of the nanoparticles is tuned by using organic dye acceptors directly conjugated onto the nanoparticle surface.

Keywords: Förster resonance energy transfer (FRET); cancer; fluorescence; fluorescence lifetime imaging microscopy (FLIM); quantum dots.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fluorescence Resonance Energy Transfer / methods
HeLa Cells
Humans
Microscopy, Fluorescence* / methods
Quantum Dots / chemistry*
Silicon / chemistry*
Surface Properties

Substances

Silicon