High-throughput bacterial functional genomics in the sequencing era

Andrew N Gray; Byoung-Mo Koo; Anthony L Shiver; Jason M Peters; Hendrik Osadnik; Carol A Gross

doi:10.1016/j.mib.2015.07.012

High-throughput bacterial functional genomics in the sequencing era

Curr Opin Microbiol. 2015 Oct:27:86-95. doi: 10.1016/j.mib.2015.07.012. Epub 2015 Sep 1.

Authors

Andrew N Gray¹, Byoung-Mo Koo¹, Anthony L Shiver², Jason M Peters¹, Hendrik Osadnik¹, Carol A Gross³

Affiliations

¹ Department of Microbiology and Immunology, University of California, San Francisco, CA 94158, USA.
² Graduate Group in Biophysics, University of California, San Francisco, CA 94158, USA.
³ Department of Microbiology and Immunology, University of California, San Francisco, CA 94158, USA; Department of Cell and Tissue Biology, University of California, San Francisco, CA 94158, USA. Electronic address: [email protected].

Abstract

High-throughput functional genomic technologies are accelerating progress in understanding the diversity of bacterial life and in developing a systems-level understanding of model bacterial organisms. Here we highlight progress in deep-sequencing-based functional genomics, show how whole genome sequencing is enabling phenotyping in organisms recalcitrant to genetic approaches, recount the rapid proliferation of functional genomic approaches to non-growth phenotypes, and discuss how advances are enabling genome-scale resource libraries for many different bacteria.

Publication types

Research Support, N.I.H., Extramural
Review

MeSH terms

Bacteria / genetics*
DNA Barcoding, Taxonomic / methods
Genetic Variation
Genome, Bacterial*
Genomics
High-Throughput Nucleotide Sequencing* / methods
Phenotype

Abstract

Publication types

MeSH terms

Grants and funding