Molecular Epidemiological Interpretation of the Epidemic of Extensively Drug-Resistant Tuberculosis in South Africa

E M Streicher; S L Sampson; K Dheda; T Dolby; J A Simpson; T C Victor; N C Gey van Pittius; P D van Helden; R M Warren

doi:10.1128/JCM.01414-15

Molecular Epidemiological Interpretation of the Epidemic of Extensively Drug-Resistant Tuberculosis in South Africa

J Clin Microbiol. 2015 Nov;53(11):3650-3. doi: 10.1128/JCM.01414-15. Epub 2015 Sep 2.

Authors

E M Streicher¹, S L Sampson¹, K Dheda², T Dolby³, J A Simpson³, T C Victor¹, N C Gey van Pittius¹, P D van Helden¹, R M Warren⁴

Affiliations

¹ DST/NRF Centre of Excellence for Biomedical Tuberculosis Research/SAMRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, South Africa.
² Lung Infection and Immunity Unit, Department of Medicine, University of Cape Town, Cape Town, South Africa.
³ National Health Laboratory Service, Cape Town, South Africa.
⁴ DST/NRF Centre of Excellence for Biomedical Tuberculosis Research/SAMRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, South Africa [email protected].

Abstract

We show that the interpretation of molecular epidemiological data for extensively drug-resistant tuberculosis (XDR-TB) is dependent on the number of different markers used to define transmission. Using spoligotyping, IS6110 DNA fingerprinting, and DNA sequence data, we show that XDR-TB in South Africa (2006 to 2008) was predominantly driven by the acquisition of second-line drug resistance.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antitubercular Agents / pharmacology*
Base Sequence
DNA Fingerprinting
Drug Resistance, Multiple, Bacterial / genetics*
Epidemics
Extensively Drug-Resistant Tuberculosis / epidemiology*
Extensively Drug-Resistant Tuberculosis / microbiology
Genetic Markers / genetics
Humans
Microbial Sensitivity Tests
Molecular Epidemiology / methods
Mycobacterium tuberculosis / classification
Mycobacterium tuberculosis / drug effects*
Mycobacterium tuberculosis / genetics
Sequence Analysis, DNA
South Africa / epidemiology
Tuberculosis, Pulmonary / epidemiology*
Tuberculosis, Pulmonary / microbiology

Substances

Antitubercular Agents
Genetic Markers