Evaluation of DiversiLab®, MLST and PFGE typing for discriminating clinical Enterococcus faecium isolates

Guido Werner; Carola Fleige; Bernd Neumann; Jennifer K Bender; Franziska Layer; Ingo Klare

doi:10.1016/j.mimet.2015.08.019

Evaluation of DiversiLab®, MLST and PFGE typing for discriminating clinical Enterococcus faecium isolates

J Microbiol Methods. 2015 Nov:118:81-4. doi: 10.1016/j.mimet.2015.08.019. Epub 2015 Sep 1.

Authors

Guido Werner¹, Carola Fleige¹, Bernd Neumann², Jennifer K Bender¹, Franziska Layer¹, Ingo Klare¹

Affiliations

¹ Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany.
² Robert Koch Institute, Wernigerode Branch, Wernigerode, Germany; Institute for Microbiology, Ernst-Moritz-Arndt-University, Greifswald, Germany.

PMID: 26341611
DOI: 10.1016/j.mimet.2015.08.019

Abstract

We evaluated and critically assessed the performance and discriminatory power of a rep-PCR based commercial test DiversiLab® Enterococcus kit (bioMerieux) for typing a set of 65 representative isolates of Enterococcus faecium/VRE and compared it to state-of-the-art typing techniques such as PFGE and MLST.

Keywords: DiversiLab; Outbreak; Rep-PCR; Typing; VRE.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Electrophoresis, Gel, Pulsed-Field / methods
Enterococcus faecium / classification*
Enterococcus faecium / genetics*
Enterococcus faecium / isolation & purification
Gram-Positive Bacterial Infections / epidemiology
Gram-Positive Bacterial Infections / microbiology*
Humans
Molecular Epidemiology / methods
Molecular Typing / methods*
Multilocus Sequence Typing / methods
Polymerase Chain Reaction / methods