Developmental expression and function analysis of protein tyrosine phosphatase receptor type D in oligodendrocyte myelination

Neuroscience. 2015 Nov 12:308:106-14. doi: 10.1016/j.neuroscience.2015.08.062. Epub 2015 Sep 1.

Abstract

Receptor protein tyrosine phosphatases (RPTPs) are extensively expressed in the central nervous system (CNS), and have distinct spatial and temporal patterns in different cell types during development. Previous studies have demonstrated possible roles for RPTPs in axon outgrowth, guidance, and synaptogenesis. In the present study, our results revealed that protein tyrosine phosphatase, receptor type D (PTPRD) was initially expressed in mature neurons in embryonic CNS, and later in oligodendroglial cells at postnatal stages when oligodendrocytes undergo active axonal myelination process. In PTPRD mutants, oligodendrocyte differentiation was normal and a transient myelination delay occurred at early postnatal stages, indicating the contribution of PTPRD to the initiation of axonal myelination. Our results also showed that the remyelination process was not affected in the absence of PTPRD function after a cuprizone-induced demyelination in adult animals.

Keywords: axonal myelination; corpus callosum; cuprizone; spinal cord.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Brain / growth & development*
  • Brain / pathology
  • Brain / physiology*
  • Brain / physiopathology
  • Cell Differentiation / physiology
  • Cuprizone
  • Demyelinating Diseases / pathology
  • Demyelinating Diseases / physiopathology
  • Disease Models, Animal
  • Evoked Potentials, Motor / physiology
  • Immunohistochemistry
  • In Situ Hybridization
  • Mice, Transgenic
  • Motor Activity / physiology
  • Mutation
  • Oligodendroglia / pathology
  • Oligodendroglia / physiology*
  • Oligodendroglia / ultrastructure
  • Receptor-Like Protein Tyrosine Phosphatases, Class 2 / genetics
  • Receptor-Like Protein Tyrosine Phosphatases, Class 2 / metabolism*
  • Spinal Cord / growth & development*
  • Spinal Cord / pathology
  • Spinal Cord / physiology*
  • Spinal Cord / physiopathology
  • Transcranial Magnetic Stimulation

Substances

  • Cuprizone
  • Ptprd protein, mouse
  • Receptor-Like Protein Tyrosine Phosphatases, Class 2