Probiotics Clostridium butyricum and Bacillus subtilis ameliorate intestinal tumorigenesis

Future Microbiol. 2015;10(9):1433-45. doi: 10.2217/fmb.15.66. Epub 2015 Sep 7.

Abstract

Aims: To investigate the antitumor effects of probiotics Clostridium butyricum and Bacillus subtilis on colorectal cancer (CRC) progression.

Materials & methods: The effects of C. butyricum and B. subtilis on CRC cells were studied. Male C57BL/6 mice with 1,2-dimethylhydrazine dihydrochloride (DMH)-induced CRC were intervened by these two probiotics and the antitumor effects were examined by comparing the tumor incidence and detecting the inflammatory and immune-related markers.

Results & conclusions: C. butyricum and B. subtilis inhibited the proliferation of CRC cells, caused cell cycle arrest and promoted apoptosis. In vivo, these two probiotics inhibited the development of DMH-induced CRC. The molecular mechanism involved reduced inflammation and improved immune homeostasis. This work establishes a basis for the protective role of probiotics B. subtilis and C. butyricum in intestinal tumorigenesis.

Keywords: colorectal cancer; gut microbiota; immunity; inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1,2-Dimethylhydrazine
  • Administration, Oral
  • Animals
  • Apoptosis
  • Bacillus subtilis / physiology*
  • Carcinogenesis*
  • Cell Cycle Checkpoints
  • Cell Line, Tumor
  • Clostridium butyricum / physiology*
  • Colorectal Neoplasms / chemically induced
  • Colorectal Neoplasms / prevention & control*
  • Culture Media, Conditioned
  • Disease Progression
  • Homeostasis
  • Inflammation / therapy
  • Male
  • Mice, Inbred C57BL
  • Probiotics*

Substances

  • Culture Media, Conditioned
  • 1,2-Dimethylhydrazine