Expression of LC3, LAMP2, KEAP1 and NRF2 in Salivary Adenoid Cystic Carcinoma

Pathol Oncol Res. 2016 Jan;22(1):109-14. doi: 10.1007/s12253-015-9981-0. Epub 2015 Sep 8.

Abstract

Salivary Adenoid Cystic Carcinoma (SACC) is a tumor characterized by inevitable local progression and terminal hematogenous metastasis. This study aimed to investigate the expression of LC3, LAMP2, KEAP1 and NRF2 in SACC. Human salivary gland tissue microarray which contains 74 SACC, 12 pleomorphic adenoma and 18 normal salivary gland specimens. High expression of LC3, LAMP2, KEAP1 and NRF2 were found in SACC patients, and LC3, LAMP2, KEAP1 and NRF2 expression were significantly higher in SACC than as compared with pleomorphic adenoma and (or) normal salivary gland. The expression of NRF2 was correlated with pathological type of human SACC (P < 0.05). Moreover, the high-expression of KEAP1 had significant correlations with LC3 (P < 0.001, R = 0.3195), and LAMP2 (P < 0.001, R = 0.3346) and NRF2 (P < 0.05, R = 0.2246) by using the Pearson correlation coefficient test. Our findings demonstrated that up-regulation of LC3, LAMP2, KEAP1 and NRF2 were associated with carcinogenesis and progression of SACC patients, suggesting that they may be useful molecular targets in salivary adenoid cystic carcinoma.

Keywords: KEAP1; LAMP2; LC3; NRF2; Salivary adenoid cystic carcinoma.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoma, Pleomorphic / metabolism*
  • Adenoma, Pleomorphic / pathology
  • Biomarkers, Tumor / metabolism
  • Carcinoma, Adenoid Cystic / metabolism*
  • Carcinoma, Adenoid Cystic / pathology
  • Case-Control Studies
  • Cohort Studies
  • Humans
  • Immunoenzyme Techniques
  • Intracellular Signaling Peptides and Proteins / metabolism*
  • Kelch-Like ECH-Associated Protein 1
  • Lysosomal-Associated Membrane Protein 2 / metabolism*
  • Microtubule-Associated Proteins / metabolism*
  • NF-E2-Related Factor 2 / metabolism*
  • Neoplasm Grading
  • Neoplasm Staging
  • Prognosis
  • Salivary Gland Neoplasms / metabolism*
  • Salivary Gland Neoplasms / pathology
  • Salivary Glands / metabolism

Substances

  • Biomarkers, Tumor
  • Intracellular Signaling Peptides and Proteins
  • KEAP1 protein, human
  • Kelch-Like ECH-Associated Protein 1
  • LAMP2 protein, human
  • Lysosomal-Associated Membrane Protein 2
  • MAP1LC3A protein, human
  • Microtubule-Associated Proteins
  • NF-E2-Related Factor 2
  • NFE2L2 protein, human