Abstract
We investigated bovine coronavirus (BCoV) as an etiological agent in cattle with clinical respiratory and digestive signs using 147 feces and 199 nasal swab samples. A total of 18 test samples (16 feces and 2 nasal swap samples) were detected positive by ELISA and/or RT-PCR targeting the BCoV N gene. The partial S1 gene regions of BCoVs (An-4 and An-11) detected in feces samples from two herd-mate dairy calves were compared. Virological and serological results indicated that BCoVs are widespread in Turkey and are likely etiological agents in diarrhea cases in calves.
Keywords:
S1 gene region; bovine; coronavirus; nested RT-PCR; sequencing.
© 2011 Blackwell Verlag GmbH.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Cattle
-
Cattle Diseases / epidemiology*
-
Cattle Diseases / virology
-
Coronavirus Infections / epidemiology
-
Coronavirus Infections / veterinary*
-
Coronavirus Infections / virology
-
Coronavirus, Bovine / genetics*
-
Coronavirus, Bovine / isolation & purification
-
Coronavirus, Bovine / metabolism
-
Enzyme-Linked Immunosorbent Assay / veterinary
-
Feces / virology
-
Molecular Sequence Data
-
Nose / virology
-
Phylogeny
-
Sequence Analysis, DNA / veterinary
-
Spike Glycoprotein, Coronavirus / genetics*
-
Spike Glycoprotein, Coronavirus / metabolism
-
Turkey / epidemiology
Substances
-
Spike Glycoprotein, Coronavirus
Associated data
-
GENBANK/GQ259978
-
GENBANK/GQ259979