Pre- and postmortem imaging of transplanted cells

Anna Andrzejewska; Adam Nowakowski; Miroslaw Janowski; Jeff W M Bulte; Assaf A Gilad; Piotr Walczak; Barbara Lukomska

doi:10.2147/IJN.S83557

Pre- and postmortem imaging of transplanted cells

Int J Nanomedicine. 2015 Sep 2:10:5543-59. doi: 10.2147/IJN.S83557. eCollection 2015.

Authors

Anna Andrzejewska¹, Adam Nowakowski¹, Miroslaw Janowski², Jeff W M Bulte³, Assaf A Gilad⁴, Piotr Walczak⁵, Barbara Lukomska¹

Affiliations

¹ NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland.
² NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland ; Department of Neurosurgery, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland ; RusselI H Morgan Department of Radiology and Radiological Science, Division of Magnetic Resonance Research, The Johns Hopkins University School of Medicine, Baltimore, MD, USA ; Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
³ RusselI H Morgan Department of Radiology and Radiological Science, Division of Magnetic Resonance Research, The Johns Hopkins University School of Medicine, Baltimore, MD, USA ; Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD, USA ; Department of Biomedical Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD, USA ; Department of Chemical & Biomolecular Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD, USA ; Department of Oncology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
⁴ RusselI H Morgan Department of Radiology and Radiological Science, Division of Magnetic Resonance Research, The Johns Hopkins University School of Medicine, Baltimore, MD, USA ; Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
⁵ RusselI H Morgan Department of Radiology and Radiological Science, Division of Magnetic Resonance Research, The Johns Hopkins University School of Medicine, Baltimore, MD, USA ; Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD, USA ; Department of Radiology, Faculty of Medical Sciences, University of Warmia and Mazury, Olsztyn, Poland.

Abstract

Therapeutic interventions based on the transplantation of stem and progenitor cells have garnered increasing interest. This interest is fueled by successful preclinical studies for indications in many diseases, including the cardiovascular, central nervous, and musculoskeletal system. Further progress in this field is contingent upon access to techniques that facilitate an unambiguous identification and characterization of grafted cells. Such methods are invaluable for optimization of cell delivery, improvement of cell survival, and assessment of the functional integration of grafted cells. Following is a focused overview of the currently available cell detection and tracking methodologies that covers the entire spectrum from pre- to postmortem cell identification.

Keywords: MRI; SPECT; bioluminescence; cell labeling; stem cells; transplantation.

Publication types

Research Support, N.I.H., Extramural
Review

MeSH terms

Animals
Autopsy*
Cell Tracking / methods*
Genes, Reporter
Humans
Luminescent Measurements
Magnetic Resonance Imaging*
Models, Animal
Stem Cell Transplantation*
Stem Cells / chemistry*
Tomography, X-Ray Computed

Abstract

Publication types

MeSH terms

Grants and funding