Sampling of Candida albicans and Candida tropicalis by Langerin-positive dendritic cells in mouse Peyer's patches

Immunol Lett. 2015 Nov;168(1):64-72. doi: 10.1016/j.imlet.2015.09.008. Epub 2015 Sep 16.

Abstract

Members of the Candida genus, including C. albicans and C. tropicalis are opportunistic fungal pathogens that are increasingly associated with gastrointestinal infections and inflammatory bowel diseases. In healthy populations, however, C. albicans and C. tropicalis are considered benign members of the mycobiome, and are presumably kept in check by the mucosal immune system. In this study, we demonstrate in mice that C. albicans and C. tropicalis are sampled by Peyer's patch (PP) dendritic cells (DCs). Uptake into gut-associated lymphoid tissues occurred rapidly and was at least partly M cell-dependent. C. albicans and C. tropicalis preferentially localized in (and persisted within) a recently identified sub- population of Peyer's patch DCs distinguished by their expression of the C-type lectin receptor, Langerin. This study is the first to identify a subset of PP DCs capable of sampling Candida species.

Keywords: C-type lectins; Dendritic cells; Diphtheria toxin; Langerin; Peyer’s patch.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Antigens, Surface / immunology*
  • Antigens, Surface / metabolism
  • Caco-2 Cells
  • Candida albicans / immunology*
  • Candida albicans / physiology
  • Candida tropicalis / immunology*
  • Candida tropicalis / physiology
  • Cell Survival / drug effects
  • Cell Survival / immunology
  • Cytokines / genetics
  • Cytokines / immunology
  • Cytokines / metabolism
  • Dendritic Cells / immunology*
  • Dendritic Cells / metabolism
  • Gene Expression / drug effects
  • Gene Expression / immunology
  • HT29 Cells
  • Host-Pathogen Interactions / drug effects
  • Host-Pathogen Interactions / immunology
  • Humans
  • Immunization / methods
  • Lectins, C-Type / immunology*
  • Lectins, C-Type / metabolism
  • Lymphoid Tissue / cytology
  • Lymphoid Tissue / immunology
  • Lymphoid Tissue / microbiology
  • Male
  • Mannose-Binding Lectins / immunology*
  • Mannose-Binding Lectins / metabolism
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Microscopy, Confocal
  • Microscopy, Electron
  • Ovalbumin / immunology
  • Ovalbumin / metabolism
  • Ovalbumin / pharmacology
  • Peyer's Patches / cytology
  • Peyer's Patches / immunology*
  • Peyer's Patches / microbiology
  • Reverse Transcriptase Polymerase Chain Reaction
  • beta-Glucans / immunology
  • beta-Glucans / metabolism
  • beta-Glucans / pharmacology

Substances

  • Antigens, Surface
  • Cd207 protein, mouse
  • Cytokines
  • Lectins, C-Type
  • Mannose-Binding Lectins
  • beta-Glucans
  • Ovalbumin