Influence of antimicrobial solutions in the decontamination and adhesion of glass-fiber posts to root canals

J Appl Oral Sci. 2015 Jul-Aug;23(4):436-41. doi: 10.1590/1678-775720150005.

Abstract

Objective: This study evaluated the effect of root canal disinfectants on the elimination of bacteria from the root canals, as well as their effect on glass-fiber posts bond strength.

Material and methods: Fifty-three endodontically treated root canals had post spaces of 11 mm in length prepared and contaminated with E. faecalis. For CFU/ml analysis, eight teeth were contaminated for 1 h or 30 days (n=4). Teeth were decontaminated with 5% NaOCl, 2% CHX, or distilled water. As control, no decontamination was conducted. After decontamination, sterile paper points were used to collect samples, and CFU/ml were counted. For push-out, three groups were evaluated (n=15): irrigation with 2.5% NaOCl, 2% CHX, or sterile distilled water. A bonding agent was applied to root canal dentin, and a glass-fiber post was cemented with a dual-cured cement. After 24 h, 1-mm-thick slices of the middle portion of root canals were obtained and submitted to the push-out evaluation. Three specimens of each group were evaluated in scanning electron microscopy (SEM). Data were analyzed with one-way ANOVA and Dunnett's T3 test (α=0.05).

Results: The number of CFU/ml increased from 1 h to 30 days of contamination in control and sterile distilled water groups. Decontamination with NaOCl was effective only when teeth were contaminated for 1 h. CHX was effective at both contamination times. NaOCl did not influence the bond strength (p>0.05). Higher values were observed with CHX (p<0.05). SEM showed formation of resin tags in all groups.

Conclusion: CHX showed better results for the irrigation of contaminated root canals both in reducing the bacterial contamination and in improving the glass-fiber post bonding.

Publication types

  • Evaluation Study

MeSH terms

  • Adhesiveness / drug effects
  • Analysis of Variance
  • Chlorhexidine / pharmacology
  • Colony Count, Microbial
  • Dental Bonding / methods
  • Dental Pulp Cavity / drug effects*
  • Dental Pulp Cavity / microbiology*
  • Dentin / drug effects
  • Dentin / microbiology
  • Dentin-Bonding Agents / chemistry
  • Disinfectants / pharmacology
  • Enterococcus faecalis / drug effects
  • Glass / chemistry*
  • Humans
  • Microscopy, Electron, Scanning
  • Post and Core Technique*
  • Resin Cements / chemistry
  • Root Canal Irrigants / pharmacology*
  • Root Canal Preparation / methods
  • Shear Strength
  • Sodium Hypochlorite / pharmacology
  • Time Factors

Substances

  • Dentin-Bonding Agents
  • Disinfectants
  • Resin Cements
  • Root Canal Irrigants
  • fiberglass
  • Sodium Hypochlorite
  • Chlorhexidine