Overexpression of Heme Oxygenase 1 Impairs Cognitive Ability and Changes the Plasticity of the Synapse

J Alzheimers Dis. 2015;47(3):595-608. doi: 10.3233/JAD-150027.

Abstract

The expression of heme oxygenase 1 (HO-1) in the cortex and hippocampus is higher in Alzheimer's disease (AD) and mild cognitive impairment patients than healthy individuals, and epidemiological studies suggest that HO-1 is an important factor for AD. However, its influence on nerve function is poorly understood. Here, we studied the effect of the overexpression of HO-1 on the cognitive and synaptic plasticity in 3-month-old mice. We found that the overexpression of HO-1 induced spatial learning and memory deficits with an apparent decrease of AMPKR, NMDAR, postsynaptic density protein 95, synapsin I, synaptophysin, and microtubule-associated protein 2, all of which are memory-related synaptic proteins. Concurrently, HO-1 could co-express and induce the aggregation of Aβ42 and Aβ oligomer in the hippocampus area. Additionally, our research is the first to demonstrate that HO-1 changes the morphology of the synapse to impair the neural circuit. These results indicate that the overexpression of HO-1 can damage synaptic plasticity in early stages to induce AD-like pathology and cognitive abnormality in mice.

Keywords: Alzheimer’s disease; amyloid-β oligomer; dendritic spine; heme oxygenase 1; synaptic plasticity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyloid beta-Peptides / metabolism
  • Animals
  • Avoidance Learning / physiology
  • Cell Line, Tumor
  • Cognition / physiology*
  • Dendrites / enzymology
  • Dendrites / pathology
  • Heme Oxygenase-1 / metabolism*
  • Hippocampus / enzymology
  • Hippocampus / pathology
  • Learning Disabilities / metabolism
  • Male
  • Maze Learning / physiology
  • Membrane Proteins / metabolism*
  • Memory Disorders / metabolism
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Neuronal Plasticity / physiology*
  • Receptors, Glutamate / metabolism
  • Synapses / enzymology*
  • Synapses / pathology

Substances

  • Amyloid beta-Peptides
  • Membrane Proteins
  • Receptors, Glutamate
  • Heme Oxygenase-1
  • Hmox1 protein, mouse