Detection of Escherichia coli sequence type 131 clonal group among extended-spectrum β-lactamase-producing E. coli using VITEK MS Plus matrix-assisted laser desorption ionization-time of flight mass spectrometry

J Microbiol Methods. 2015 Dec:119:7-9. doi: 10.1016/j.mimet.2015.09.016. Epub 2015 Sep 28.

Abstract

We investigated the performance of the VITEK MS Plus system for the detection of Escherichia coli sequence type 131 (ST131) among extended-spectrum β-lactamase-producing E. coli isolates. The SARAMIS software could discriminate the 67 ST131 isolates from 82 non-ST131 isolates with a sensitivity of 86.6% and a specificity of 95.1%.

Keywords: Clone; ESBL; MALDI-TOF MS; Sequence types.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Escherichia coli / chemistry*
  • Escherichia coli / enzymology
  • Escherichia coli / genetics
  • Escherichia coli / isolation & purification*
  • Escherichia coli Infections / microbiology*
  • Humans
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • beta-Lactamases / genetics
  • beta-Lactamases / metabolism*

Substances

  • beta-Lactamases